Integrated analysis of microRNA and mRNA expression profiles in rats with selenium deficiency and identification of associated miRNA-mRNA network

被引：17
作者：
Feng, Yanjing [1 ]
Xing, Yunjie [1 ]
Liu, Zhongwei [1 ]
Yang, Guang [1 ]
Niu, Xiaolin [1 ,2 ]
Gao, Dengfeng [1 ]
机构：
[1] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Cardiol, Sch Med, Xian, Shaanxi, Peoples R China
[2] Yanan Univ, Sch Med, Affiliated Hosp 3, Dept Cardiol,Meishan Branch, Meishan, Sichuan, Peoples R China
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
KESHAN-DISEASE; CELLS; GENES; NRF2;
D O I：
10.1038/s41598-018-24826-w
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Selenium deficiency is closely related with various type of cardiovascular disease. However, the miRNA-mRNA regulatory network in Selenium deficiency related cardiac change remains to be understand. In the present study, a reliable Selenium deficiency rat model was established and confirmed by pathological and biochemical examination. The mRNA and miRNA expression profiles were conducted by microarray technology. Gene Ontology (GO) Analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analysis was performed to investigate the function of targeted genes, and the relationship between miRNA and mRNA was studied by network analysis. A total of 4931 mRNAs and 119 miRNAs was differentially expressed between any two groups (control group, low-selenium group and selenium supplementation group). GO and KEGG pathway analysis of selected miRNAs target genes found that selenium deficiency was related to several different biological processes. Furthermore, a miRNA-mRNA regulatory network was conducted to illustrate the interaction of miRNAs and these targeted genes. In conclusion, our present study provides a new insight that potential molecular mechanism of Selenium deficiency was a multiply miRNAs and mRNA caused biological change.
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页数：9
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