P-32-postlabeling of 1,3-butadiene and 4-vinyl-1-cyclohexene metabolite DNA adducts: In vitro and in vivo applications

被引：6

作者：

Mabon, N ^{[1
]}

Randerath, K ^{[1
]}

机构：

[1] BAYLOR COLL MED,DEPT PHARMACOL,DIV TOXICOL,HOUSTON,TX 77030

来源：

TOXICOLOGY | 1996年 / 113卷 / 1-3期

关键词：

1,3-butadiene; 4-vinyl-1-cyclohexene; diene diepoxides; P-32-postlabeling; DNA adducts; mouse skin;

D O I：

10.1016/0300-483X(96)03470-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Epoxides of 1,3-butadiene, i.e. 1,2-epoxy-3-butene and 1,2:3,4-diepoxybutane are DNA-reactive metabolites. No methods have been reported for detecting DNA adducts of these compounds in exposed animals or humans. The purpose of this study has been to develop a P-32-postlabeling assay for adducts of 1,3-butadiene and its dimer, 4-vinyl-1-cyclohexene. The assay was applied to skin DNA of mice exposed topically to diepoxides of these dienes. A dose-dependent increase in in vivo adduct formation was observed for both compounds. The newly developed assay will find applications in mechanistic studies on these and related compounds and in human biomonitoring.

引用

页码：341 / 344

页数：4

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