Construction of three single-chain antibody fragment variable fusion structures for sensitive detection of nucleocapsid protein of Hantaan virus

被引：2

作者：

Chen, Jia

Xie, Wei-Hong

Zhang, Zhi-Ping

Zhang, Xian-En ^{[1
]}

Li, Wei

Wang, Shi-Hua

Zhou, Ya-Feng

Liang, Mi-Fang

Wang, Ke-Min

Yang, Zhan-Qiu

机构：

[1] Chinese Acad Sci, Joint Res Grp Analyt Pathogen Microbiol, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, State Key Lab Macromol, Beijing 100080, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

[4] Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China

[5] Hunan Univ, State Key Lab Chem & Biol Sensing & Chemometr, Changsha, Peoples R China

[6] Wuhan Univ, Inst Virol, Coll Med, Wuhan 430072, Peoples R China

来源：

ANALYTICAL LETTERS | 2006年 / 39卷 / 10期

关键词：

Hantaan virus; single-chain antibody fragment variable (scFv); fusion protein; alkaline phosphatase; fluorescence dye; nanoparticles;

D O I：

10.1080/00032710600748905

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Three single-chain fragment variable (scFv) fusion structures were constructed for use in rapid and sensitive detection of nucleocapsid protein (NP) of Hantaan virus. The detection of NPs on glass chips was signalized by enzyme labeling or fluorescence dye Cy3, or Cy5 cluster nanoparticles. The sensitivity of the methods with different signal systems was evaluated and compared. The detection limits of scFv-alkaline phosphatase fusion, fluorescence labeling (scFv-Cy3), and nanoparticles labeling (scFv-SBP-streptavidin-nanoparticle) were 0.1 mu g/mL, 1 ng/mL, and 0.1 ng/mL NP, respectively, which were all lower than that in a conventional enzyme-linked immunosorbent assay (ELISA) (1 mu g/mL). Twenty Hantaan virus isolates were detected using the proposed methods.

引用

页码：2153 / 2167

页数：15

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