Different output properties of perisomatic region-targeting interneurons in the basal amygdala

被引:18
作者
Barsy, Boglarka [1 ]
Szabo, Gergely G. [1 ]
Andrasi, Tibor [1 ,2 ]
Vikor, Attila [1 ]
Hajos, Norbert [1 ]
机构
[1] Hungarian Acad Sci, Inst Expt Med, Lendulet Lab Network Neurophysiol, H-1450 Budapest, Hungary
[2] Semmelweis Univ, Janos Szentagothai Sch Neurosci, Budapest, Hungary
关键词
firing property; GABAergic circuits; inhibition; mouse; synaptic transmission; RAT BASOLATERAL AMYGDALA; PARVALBUMIN-IMMUNOREACTIVE INTERNEURONS; CHOLINERGIC-RECEPTOR ACTIVATION; IN-VIVO; BASKET CELLS; PYRAMIDAL CELLS; POSITIVE INTERNEURONS; GABAERGIC INTERNEURONS; INHIBITORY SYNAPSES; TRANSMITTER RELEASE;
D O I
10.1111/ejn.13498
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The perisomatic region of principal neurons in cortical regions is innervated by three types of GABAergic interneuron, including parvalbumin-containing basket cells (PVBCs) and axo-axonic cells (AACs), as well as cholecystokinin and type 1 cannabinoid receptor-expressing basket cells (CCK/CB1BCs). These perisomatic inhibitory cell types can also be found in the basal nucleus of the amygdala, however, their output properties are largely unknown. Here, we performed whole-cell recordings in morphologically identified interneurons in slices prepared from transgenic mice, in which the GABAergic cells could be selectively targeted. Investigating the passive and active membrane properties of interneurons located within the basal amygdala revealed that the three interneuron types have distinct single-cell properties. For instance, the input resistance, spike rate, accommodation in discharge rate, or after-hyperpolarization width at the half maximal amplitude separated the three interneuron types. Furthermore, we performed paired recordings from interneurons and principal neurons to uncover the basic features of unitary inhibitory postsynaptic currents (uIPSCs). Although we found no difference in the magnitude of responses measured in the principal neurons, the uIPSCs originating from the distinct interneuron types differed in rise time, failure rate, latency, and short-term dynamics. Moreover, the asynchronous transmitter release induced by a train of action potentials was typical for the output synapses of CCK/CB1BCs. Our results suggest that, despite the similar uIPSC magnitudes originating from the three perisomatic inhibitory cell types, their distinct release properties together with the marked differences in their spiking characteristics may contribute to accomplish specific functions in amygdala network operation.
引用
收藏
页码:548 / 558
页数:11
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