Effects of Excess Copper Ions on Decidualization of Human Endometrial Stromal Cells

被引：9
作者：
Li, Ying [1 ,2 ]
Kang, Zhen-Long [1 ]
Qiao, Na [1 ]
Hu, Lian-Mei [1 ]
Ma, Yong-Jiang [1 ]
Liang, Xiao-Huan [1 ]
Liu, Ji-Long [1 ]
Yang, Zeng-Ming [1 ]
机构：
[1] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
[2] Guangdong Prov Key Lab Prevent & Control Severe C, Guangzhou 510642, Guangdong, Peoples R China
来源：
BIOLOGICAL TRACE ELEMENT RESEARCH | 2017年 / 177卷 / 01期
基金：
美国国家科学基金会;
关键词：
Human endometrial stromal cells; In vitro decidualization; Oxidative stress; Copper; INTRAUTERINE-DEVICE USERS; IN-VITRO DECIDUALIZATION; FACTOR-BINDING PROTEIN-1; OXIDATIVE STRESS; HEAVY-METALS; IMPLANTATION; EXPRESSION; PREGNANCY; PROLIFERATION; COMPLEXES;
D O I：
10.1007/s12011-016-0840-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study was to investigate the effects of copper ions on decidualization of human endometrial stromal cells (HESCs) cultured in vitro. Firstly, non-toxic concentrations of copper D-gluconate were screened in HESCs based on cell activity. Then, the effects of non-toxic concentrations of copper ions (0 similar to 250 mu M) were examined on decidualization of human endometrial stromal cells. Our data demonstrated that the mRNA expressions of insulin-like growth factor binding protein (IGFBP-1), prolactin (PRL), Mn-SOD, and FOXO1were down-regulated during decidualization following the treatments with 100 or 250 mu M copper ions. Meanwhile, the amount of malonaldehyde (MDA) in the supernatant of HESCs was increased. These results showed that in vitro decidualization of HESCs was impaired by copper treatment.
引用
收藏
页码：10 / 15
页数：6
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