Application of the yeast two-hybrid system in molecular gerontology

被引：8

作者：

Knudsen, CR ^{[1
]}

Jadidi, M ^{[1
]}

Friis, I ^{[1
]}

Mansilla, F ^{[1
]}

机构：

[1] Aarhus Univ, Inst Mol & Struct Biol, DK-8000 Aarhus C, Denmark

来源：

BIOGERONTOLOGY | 2002年 / 3卷 / 04期

关键词：

ageing; Alzheimer's disease; apoptosis; cellular senescence; protein-protein interactions; protein repair; telomere shortening; two-hybrid system; Werner's syndrome;

D O I：

10.1023/A:1016219121897

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Most - if not all - proteins are bound to interact with other proteins to exert their function, and thus the identification of the interaction partners of a protein is vital in proteomics. The yeast two-hybrid system is a popular and effective tool for studying protein-protein interactions. Although the advantages of the system are manifold, it also has certain drawbacks and limitations. The two-hybrid system has been shown to be extremely useful for placing a protein of unknown function within a functional context, thereby providing information about a putative role of the uncharacterised protein. This concept has also been successfully applied in molecular gerontology.

引用

页码：243 / 256

页数：14

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