Effect of cadmium in the clam Ruditapes decussatus assessed by proteomic analysis

被引:93
作者
Chora, Suze [1 ,2 ]
Starita-Geribaldi, Mireille [3 ]
Guigonis, Jean-Marie [4 ]
Samson, Michel [4 ,5 ]
Romeo, Michele [2 ]
Bebianno, Maria Joao [1 ]
机构
[1] Univ Algarve, CIMA, Fac Marine & Environm Sci, P-8000139 Faro, Portugal
[2] Univ Nice Sophia Antipolis, EA 4228, ECOMERS, F-06108 Nice 2, France
[3] Univ Nice Sophia Antipolis, Lab Oral Microbiol, URE 01, Fac Odontol,Pole Univ St Jean dAngely, F-06357 Nice 4, France
[4] Univ Nice Sophia Antipolis, Plateforme Bernard Rossi Proteome Metabolome IFR5, F-06107 Nice, France
[5] INSERM, U638, F-06107 Nice, France
关键词
Cadmium; Proteomic; Ruditapes decussatus; LC-MS/MS; LAGOON-OF-VENICE; MYTILUS-GALLOPROVINCIALIS; OXIDATIVE-STRESS; ACTIN CYTOSKELETON; LIPID-PEROXIDATION; PROTEIN EXPRESSION; MYA-ARENARIA; TRACE-METALS; METALLOTHIONEIN; IDENTIFICATION;
D O I
10.1016/j.aquatox.2009.07.014
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Cadmium, an environmental stressor due to its toxicity, persistence and accumulation in biota, is widespread in the aquatic environment. Cadmium accumulation kinetics have revealed that Ruditapes decussatus has a high affinity to this metal. Proteomics is an effective tool to evaluate the toxic effects of contaminants. The aim of this study was to investigate the Cd effects in the gill and digestive gland of the sentinel species R. decussatus. Protein expression profiles (PEPs) in the clam tissues exposed to Cd (40 mu g l(-1), 21 days) were compared to unexposed ones. Cd induces major changes in tissue-specific protein expression profiles in gill and digestive gland. This tissue dependent response results mainly from differences in Cd accumulation, protein inhibition and/or autophagy. An overall decrease of protein spots was detected in both treated tissues, being higher in gill. Some of the spots more drastically altered after pollutants exposure were excised and ninewere identified by micro liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins identified by homology search in databases included: three proteins (8-fold) up-regulated, one down-regulated, four suppressed and one induced. Cd induces major changes in proteins involved in cytoskeletal structure maintenance (muscle-type actin, adductor muscle actin and beta-tubulin), cell maintenance (Rab GDP) and metabolism (ALDH and MCAD, both identified by de novo sequencing) suggesting potential energetic change. They provide a valuable knowledge of Cd effects at biochemical and molecular levels in the gill and digestive gland of R. decussatus. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:300 / 308
页数:9
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