Electrochemistry and biosensing of glucose oxidase immobilized on Pt-dispersed mesoporous carbon

被引：27
|
作者：
You, Chunping [1 ,2 ,3 ]
Li, Xiang [1 ,2 ]
Zhang, Song [1 ,2 ]
Kong, Jilie [1 ,2 ]
Zhao, Dongyuan [1 ,2 ]
Liu, Baohong [1 ,2 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai Key Lab Mol Catalysis & Novel Mat, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Adv Mat & Biomed Sci, Shanghai 200433, Peoples R China
[3] Bright Dairy & Food Co Ltd, Ctr Technol, Shanghai 200436, Peoples R China
来源：
MICROCHIMICA ACTA | 2009年 / 167卷 / 1-2期
关键词：
Glucose oxidase; Biosensor; Electron transfer; Mesoporous carbon; Nanoparticles; DIRECT ELECTRON-TRANSFER; PLATINUM NANOPARTICLES; HYDROGEN-PEROXIDE; PASTE ELECTRODES; NANOTUBES; LAYER; FABRICATION; MATRIX; FILM; BIOCATALYSIS;
D O I：
10.1007/s00604-009-0228-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for immobilizing proteins in a carbon mesoporous material (CMM) containing platinum nanoparticles (Pt-NPs) is demonstrated. Compared to pure CMM or carbon nanotubes, CMM containing Pt-NPs enhances the electron transfer and redox properties of redox enzymes, such as glucose oxidase (GOx), due to a cooperative effect of Pt-NPs and CMM. The quasi-reversible electron transfer of GOx in this system is probed, and the apparent heterogeneous electron transfer rate constants are found to be 66% larger than in pure CMM. The GOx/Pt-CMM based glucose biosensor enables the determination of glucose at a potential of 600 mV (vs. SCE). Its detection limit is 10 times lower, and the sensitivity is 16 times higher than that of the respective biosensor without Pt-NPs.
引用
收藏
页码：109 / 116
页数：8
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