FGF2 stimulates the proliferation of human mesenchymal stem cells through the transient activation of JNK signaling

被引：84
作者：
Ahn, Hee-Jin [1 ]
Lee, Woo-Jung [1 ]
Kwack, KyuBum [1 ]
Do Kwon, Young [1 ]
机构：
[1] CHA Univ, Grad Sch, Dept Biomed Sci, Gyeonggi Do 487010, South Korea
来源：
FEBS LETTERS | 2009年 / 583卷 / 17期
关键词：
Mesenchymal stem cell; FGF2; c-Jun N-terminal kinase; Proliferation; Differentiation potential; N-TERMINAL KINASE; MARROW STROMAL CELLS; FIBROBLAST-GROWTH-FACTOR-2; MECHANISMS; APOPTOSIS;
D O I：
10.1016/j.febslet.2009.07.056
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
FGF2 has been shown to enhance proliferation and maintain differentiation potential in hMSCs during in vitro propagation. In this study, we investigated the role of mitogen-activated protein kinase in the functions of FGF2 in hMSCs. We demonstrated that FGF2 induces the transient activation of c-Jun N-terminal kinase (JNK), but not extracellular signal-regulated protein kinase or p38 protein kinase. SP600125 and a dominant negative JNK1 significantly reduced the FGF2-enhanced proliferation of hMSCs. Treatment with SP600125 also diminished the activity of FGF2 in the maintenance of adipogenic and osteogenic differentiation potential. These results suggest that JNK signaling is involved in the FGF2-induced stimulation of the proliferation and the maintenance of differentiation potential in hMSCs. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2922 / 2926
页数：5
相关论文
共 24 条
[1]   Inflammatory pathway analysis using a high content screening platform [J].
Bertelsen, M ;
Sanfridson, A .
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2005, 3 (03) :261-271
[2]   Ex vivo enrichment of mesenchymal cell progenitors by fibroblast growth factor 2 [J].
Bianchi, G ;
Banfi, A ;
Mastrogiacomo, M ;
Notaro, R ;
Luzzatto, L ;
Cancedda, R ;
Quarto, R .
EXPERIMENTAL CELL RESEARCH, 2003, 287 (01) :98-105
[3]   Core transcriptional regulatory circuitry in human embryonic stem cells [J].
Boyer, LA ;
Lee, TI ;
Cole, MF ;
Johnstone, SE ;
Levine, SS ;
Zucker, JR ;
Guenther, MG ;
Kumar, RM ;
Murray, HL ;
Jenner, RG ;
Gifford, DK ;
Melton, DA ;
Jaenisch, R ;
Young, RA .
CELL, 2005, 122 (06) :947-956
[4]   The role of c-Jun N-terminal kinase (JNK) in apoptosis induced by ultraviolet C and gamma radiation - Duration of JNK activation may determine cell death and proliferation [J].
Chen, YR ;
Wang, XP ;
Templeton, D ;
Davis, RJ ;
Tan, TH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (50) :31929-31936
[5]   Mechanisms underlying differential responses to FGF signaling [J].
Dailey, L ;
Ambrosetti, D ;
Mansukhani, A ;
Basilico, C .
CYTOKINE & GROWTH FACTOR REVIEWS, 2005, 16 (02) :233-247
[6]   Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli [J].
Di Nicola, M ;
Carlo-Stella, C ;
Magni, M ;
Milanesi, M ;
Longoni, PD ;
Matteucci, P ;
Grisanti, S ;
Gianni, AM .
BLOOD, 2002, 99 (10) :3838-3843
[7]   Fibroblast growth factor signaling in embryonic and cancer stem cells [J].
Dvorak, Petr ;
Dvorakova, Dana ;
Hampl, Ales .
FEBS LETTERS, 2006, 580 (12) :2869-2874
[8]   FGF-2 suppresses cellular senescence of human mesenchymal stem cells by down-regulation of TGF-β2 [J].
Ito, Tomomi ;
Sawada, Rumi ;
Fujiwara, Yoko ;
Seyama, Yousuke ;
Tsuchiya, Toshie .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 359 (01) :108-114
[9]   Smad3-dependent nuclear translocation of β-catenin is required for TGF-β1-induced proliferation of bone marrow-derived adult human mesenchymal stem cells [J].
Jian, HY ;
Shen, X ;
Liu, I ;
Sernenov, M ;
He, X ;
Wang, XF .
GENES & DEVELOPMENT, 2006, 20 (06) :666-674
[10]   Role of c-jun N-terminal kinase in the PDGF-induced proliferation and migration of human adipose tissue-derived mesenchymal stem cells [J].
Kang, YJ ;
Jeon, ES ;
Song, HY ;
Woo, JS ;
Jung, JS ;
Kim, YK ;
Kim, JH .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2005, 95 (06) :1135-1145
123