One step synthesis of functionalized carbon dots for the ultrasensitive detection of Escherichia coli and iron (III)

被引:92
作者
Chandra, Soumen [1 ]
Mahto, Triveni Kumar [1 ]
Chowdhuri, Angshuman Ray [1 ]
Das, Balaram [2 ]
Sahu, Sumanta Kumar [1 ]
机构
[1] Indian Inst Technol ISM, Dept Appl Chem, Dhanbad 826004, Jharkhand, India
[2] Vidyasagar Univ, Dept Human Physiol Community Hlth, Immunol & Microbiol Lab, Midnapore 721102, India
关键词
Carbon dots; Solid phase synthesis; Colistin; Fe3+ ion detection; E. coil detection; GRAPHENE QUANTUM DOTS; E; COLI; FLUORESCENT-PROBE; SENSITIVE DETECTION; FACILE SYNTHESIS; RAPID DETECTION; NITROGEN; FE3+; BACTERIA; O157H7;
D O I
10.1016/j.snb.2017.02.017
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper, we report a single step solid phase synthesis strategy for colistin modified fluorescent carbon dots (CDs@colistin) for the selective detection of gram-negative bacteria like Escherichia coli. Colistin is a well known cyclic polypeptide antibiotic drug but here we have used as a binding ligand for E. coli. Here also, the synthesised CDs@colistin are used for selective Fe3+ metal ion detection. The detection limits are 460 cfu/mL for E. coli and 56 nM for Fe3+ with a linear range of 3.81 x 10(2)-2.44 x 10(4) cfu/mL and 0-48 mu M, respectively. The synthesised CDs@colistin are well dispersed in water with an uniform particle size ranging from 2 nm to 5 nm executing high quantum yield of 7.56%. The synthesis process of CDs@colistin is simple, cheap, and less time consuming. Simultaneously, we have applied CDs@colistin as a novel probe for E. coil detection in three different real samples like human urine, apple juice, and tap water to evaluate the practical applicability of the synthesised colistin conjugated carbon dots. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:835 / 844
页数:10
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