A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY

被引：4

作者：

Gombault, Aurelie ^{[1
,2
]}

Warringer, Jonas ^{[3
]}

Caesar, Robert ^{[3
]}

Godin, Fabienne ^{[1
,2
]}

Vallee, Beatrice ^{[1
,2
]}

Doudeau, Michel ^{[1
,2
]}

Chautard, Helene ^{[4
]}

Blomberg, Anders ^{[3
]}

Benedetti, Helene ^{[1
,2
]}

机构：

[1] Univ Orleans, Ctr Biophys Mol, UPR 4301, CNRS, F-45071 Orleans 2, France

[2] INSERM, Orleans, France

[3] Univ Gothenburg, Dept Cell & Mol Biol, Lundberg Lab, Gothenburg, Sweden

[4] Genethon, Evry, France

来源：

FEMS YEAST RESEARCH | 2009年 / 9卷 / 06期

关键词：

yeast phenotypic study; Ras/cAMP/PKA pathway; vacuolar protease; stress response; diauxic shift; PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN; SACCHAROMYCES-CEREVISIAE; CARBOXYPEPTIDASE-Y; I-C; TERMINAL ACETYLTRANSFERASE; COGNATE PROTEINASE; YEAST; KINASE; FAMILY; SUPPRESSOR;

D O I：

10.1111/j.1567-1364.2009.00535.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase-activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.

引用

页码：867 / 874

页数：8

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