Waste recombinant DNA: Effectiveness of thermo-treatment to manage potential gene pollution

被引：3

作者：

Fu, Xiaohua ^{[1
]}

Li, Mengnan ^{[1
]}

Zheng, Guanghong ^{[1
]}

Le, Yiquan ^{[1
]}

Wang, Lei ^{[1
]}

机构：

[1] Tongji Univ, Sch Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2009年 / 157卷 / 8-9期

关键词：

Decay; Gene diffusion; Plasmid DNA; Thermo-treatment; Transformation; TRANSGENIC PLANTS; EXTRACELLULAR DNA; RESISTANCE GENES; PERSISTENCE; TRANSFORMATION; ENVIRONMENT; BIOSAFETY; BACTERIA;

D O I：

10.1016/j.envpol.2009.03.003

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Heating at 100 degrees C for 5-10 min is a common method for treating wastewater containing recombinant DNA in many bio-laboratories in China. In this experiment, plasmid pET-28b was used to investigate decay efficiency of waste recombinant DNA during thermo-treatment. The results showed that the decay half-life of the plasmid was 2.7-4.0 min during the thermo-treatment, and even heating for 30 min the plasmids still retained some transforming activity. Low pH promoted the decay of recombinant DNA, but NaCl, bovine serum albumin and EDTA, which existed in the most wastewater from bio-laboratories, protected DNA from degradation. Thus, the decay half-life of plasmid DNA may be longer than 2.7-4.0 min practically. These results suggest that the effectiveness of heating at 100 degrees C for treating waste recombinant DNA is low and a gene pollution risk remains when those thermo-treated recombinant DNAs are discharged into the environment. Therefore other simple and effective methods should be developed. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2536 / 2541

页数：6

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