miR-375 Suppresses IGF1R Expression and Contributes to Inhibition of Cell Progression in Laryngeal Squamous Cell Carcinoma

被引:45
作者
Luo, Jie [1 ]
Wu, Jianhui [1 ]
Li, Zenghong [2 ]
Qin, Hao [3 ]
Wang, Bin [1 ]
Wong, Thian-Sze [2 ]
Yang, Weiqiang [1 ]
Fu, Qing-Ling [4 ]
Lei, Wenbin [1 ]
机构
[1] Sun Yat Sen Univ, Otorhinolaryngol Inst, Affiliated Hosp 1, Dept Otorhinolaryngol, Guangzhou 510275, Guangdong, Peoples R China
[2] Univ Hong Kong, Queen Mary Hosp, Dept Surg, Hong Kong, Hong Kong, Peoples R China
[3] First Peoples Hosp Foshan, Dept Otorhinolaryngol, Foshan, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 1, Otorhinolaryngol Hosp, Guangzhou 510080, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
GROWTH-FACTOR RECEPTOR; DOWN-REGULATION; CANCER; HEAD; METASTASIS; PATHWAY; MIGRATION; INVASION;
D O I
10.1155/2014/374598
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
MicroRNAs (miRNAs) are small noncoding RNA molecules which are involved in tumorigenesis and development. To investigate their role in primary laryngeal squamous cell carcinoma (LSCC), miRNA GeneChips were used to screen the differentially expressed miRNA, and then validated by real-time quantitative PCR in LSCC samples, we found that miR-375 was frequently downregulated in primary LSCC tissues. The tumor-suppressive effect of miR-375 was determined by in vitro assays; through gain-of-function studies we demonstrated that miR-375 can inhibit LSCC cell (SNU-48 and SNU-899) proliferation, motility, and invasion, and promote their apoptosis. In addition, bioinformatics tools TargetScan, PicTar, and Miranda were used to investigate the potential target of miR-375; bioinformatics analysis and dual-luciferase reporter assay indicated that IGF1R was a novel direct target of miR-375. Ectopic transfection of miR-375 led to a significant reduction in IGF1R and its downstream signaling molecule AKT at both the mRNA and protein levels in LSCC cells. Our results suggested that downregulation of miR-375 is one of the molecular mechanisms for the development and progression of LSCC by directly targeting IGF1R and affecting its downstream AKT signaling pathways. Furthermore, miR-375 and IGF1R may serve as a novel therapeutic target for LSCC.
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页数:11
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