Molecular mass distribution of sodium alginate by high-performance size-exclusion chromatography

被引：51
|
作者：
Ci, SX [1 ]
Huynh, TH [1 ]
Louie, LW [1 ]
Yang, A [1 ]
Beals, BJ [1 ]
Ron, N [1 ]
Tsang, WG [1 ]
Soon-Shiong, P [1 ]
Desai, NP [1 ]
机构：
[1] VivoRx Inc, Santa Monica, CA 90404 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1999年 / 864卷 / 02期
关键词：
molecular mass distribution; sodium alginate; dextran; pullulan; polysaccharides;
D O I：
10.1016/S0021-9673(99)01029-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive high-performance size-exclusion chromatography (HPSEC) method with simple UV detection was developed for the molecular mass analysis of sodium alginate. It was used to evaluate alginates of varying molecular mass and the results were compared with the viscosity measurements. This HPSEC method was sensitive to serve as the stability indicating method for alginate after storage under different conditions. The information of relative molecular mass distribution of alginate was provided with reference to pullulan molecular mass standards. The comparison of the HPSEC chromatograms of alginate, pullulan and dextran revealed the effect of chemical composition of a polysaccharide and its effect on apparent molecular mass distribution. (C) 1999 Elsevier Science B.V. All rights reserved.
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页码：199 / 210
页数：12
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