Crystallization and preliminary X-ray studies of mouse tumor necrosis factor

被引：2
|
作者：
Baeyens, KJ
DeBondt, HL
Raeymaekers, A
DeRanter, CJ
Fiers, W
机构：
[1] VIB,MOL BIOL LAB,B-9000 GHENT,BELGIUM
[2] STATE UNIV GHENT,B-9000 GHENT,BELGIUM
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1997年 / 53卷
关键词：
D O I：
10.1107/S090744499601534X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Well diffracting crystals of recombinant mouse tumor necrosis factor (mTNF) have been obtained. The sitting-drop vapor-diffusion method was used to grow crystals suitable for X-ray studies from solutions containing methoxypolyethylene glycol 2000 and isopropanol as precipitants. The crystals belong to the space group P1 with unit-cell dimensions a = 49.40, b = 48.24, c = 51.13 Angstrom, alpha = 115.06, beta = 103.32, gamma = 91.27 degrees and one trimer in the asymmetric unit. Crystals are stable to X-rays and diffract beyond 2.0 Angstrom. Cooling the crystal during data collection is necessary since crystals dissolve at room temperature.
引用
收藏
页码：329 / 330
页数：2
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