A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex-Peptide Monomers by Flow Cytometry

被引：3

作者：

Chandran, P. Anoop ^{[1
,2
]}

Heidu, Sonja ^{[1
,2
]}

Zelba, Henning ^{[1
,2
]}

Schmid-Horch, Barbara ^{[3
]}

Rammensee, Hans-Georg ^{[1
,2
]}

Pascolo, Steve ^{[4
]}

Gouttefangeas, Cecile ^{[1
,2
]}

机构：

[1] Eberhard Karls Univ Tubingen, Interfac Inst Cell Biol, Dept Immunol, Tubingen, Germany

[2] German Canc Consortium DKTK, German Canc Res Ctr DKFZ Partner Site Tuebinge, Partner Site Tuebingen, Tubingen, Germany

[3] Univ Hosp, Ctr Clin Transfus Med GmbH, Tubingen, Germany

[4] Univ Hosp, Dept Dermatol, Zurich, Switzerland

来源：

FRONTIERS IN IMMUNOLOGY | 2017年 / 8卷

关键词：

MHC-peptide monomers; multimers; antigen-specific T cells; quality control; UV-peptide exchange; CLASS-I MOLECULES; MONOCLONAL-ANTIBODY; T-CELLS; MHC MULTIMERS; HEAVY-CHAINS; ANTIGEN; EPITOPE; EXCHANGE; HLA-A2;

D O I：

10.3389/fimmu.2017.00096

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage.

引用

页数：8

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