Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV

被引：26

作者：

Chen, Changmu ^{[1
]}

Cui, Shangjin ^{[1
]}

Zhang, Chaofan ^{[1
]}

Li, Jun ^{[2
]}

Wang, Jinbao ^{[2
]}

机构：

[1] Harbin Vet Res Inst CAAS, Div Swine Infect Dis, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China

[2] Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

来源：

VIRUS GENES | 2010年 / 40卷 / 01期

关键词：

Porcine reproductive and respiratory syndrome virus; Reverse-transcription loop-mediated isothermal amplification; RESPIRATORY SYNDROME VIRUS; PORCINE EPIDEMIC ABORTION; RAPID DETECTION; EXPERIMENTAL REPRODUCTION; LELYSTAD VIRUS; ANTIBODIES; DISEASE; ASSAY; PARVOVIRUS; CIRCOVIRUS;

D O I：

10.1007/s11262-009-0419-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV), four primers specific to six regions of the N gene were designed. After amplification in an isothermal water bath for 1 h, samples containing PRRSV generated the expected ladder-like products while porcine parvovirus, porcine circovirus, classic swine fever virus, pseudorabies virus, and swine testis cells generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Because it is specific and simple, the RT-LAMP assay will be useful for the diagnosis of PRRSV infection.

引用

页码：76 / 83

页数：8

共 29 条

[1]

ALBERT R, 2009, J VET DIAGN INVEST, V21, P350

[2] Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: Coexistence of two NA-subgenotypes with great diversity [J].