Role of Escherichia coli Hfq in late-gene silencing of bacteriophage T4 dmd mutant

被引:6
作者
Ueno, H [1 ]
Yonesaki, T [1 ]
机构
[1] Osaka Univ, Grad Sch Sci, Dept Biol, Osaka 5600043, Japan
关键词
D O I
10.1266/ggs.77.301
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When the dmd gene of bacteriophage T4 is mutated, many T4 late genes are post-transcriptionally silenced because of rapid mRNA degradation. Here we show that the host hfq gene is involved in the rapid mRNA degradation in a dmd mutant. A disruption of the hfq gene caused weak but significant effects on the stability of late-gene mRNA, the late-gene expression and the growth of a dmd mutant. By probing with the soc gene, we found that disruption of the hfq gene impaired the translation-independent mRNA degradation, one of two mechanisms promoting rapid mRNA degradation. We also showed that purified Hfq protein bound stoichiometrically to soc RNA. These results strongly suggest that the hfq gene has a stimulatory role in dmd mutant-specific mRNA degradation.
引用
收藏
页码:301 / 308
页数:8
相关论文
共 29 条
[1]   Twelve species of the nucleoid-associated protein from Escherichia coli -: Sequence recognition specificity and DNA binding affinity [J].
Azam, TA ;
Ishihama, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (46) :33105-33113
[2]  
CHRISTENSEN AC, 1984, BACTERIOPHAGE T4, P184
[3]   DECAY OF OMPA MESSENGER-RNA AND PROCESSING OF 9S-RNA ARE IMMEDIATELY AFFECTED BY SHIFTS IN GROWTH-RATE, BUT IN OPPOSITE MANNERS [J].
GEORGELLIS, D ;
ARVIDSON, S ;
VONGABAIN, A .
JOURNAL OF BACTERIOLOGY, 1992, 174 (16) :5382-5390
[4]   RNA CHAPERONES AND THE RNA FOLDING PROBLEM [J].
HERSCHLAG, D .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (36) :20871-20874
[5]  
HSU T, 1990, J BIOL CHEM, V265, P5303
[6]  
Kai T, 2002, GENETICS, V160, P5
[7]   Involvement of other bacteriophage T4 genes in the blockade of protein synthesis and mRNA destabilization by a mutation of gene 61.5 [J].
Kai, T ;
Ueno, H ;
Yonesaki, T .
VIROLOGY, 1998, 248 (01) :148-155
[8]  
Kai T, 1996, GENETICS, V144, P7
[9]   REGULATION OF THE ESCHERICHIA-COLI HFQ GENE ENCODING THE HOST FACTOR FOR PHAGE Q-BETA [J].
KAJITANI, M ;
KATO, A ;
WADA, A ;
INOKUCHI, Y ;
ISHIHAMA, A .
JOURNAL OF BACTERIOLOGY, 1994, 176 (02) :531-534
[10]   IDENTIFICATION AND SEQUENCE DETERMINATION OF THE HOST FACTOR GENE FOR BACTERIOPHAGE-Q-BETA [J].
KAJITANI, M ;
ISHIHAMA, A .
NUCLEIC ACIDS RESEARCH, 1991, 19 (05) :1063-1066