A novel peptide-based sensor platform for detection of anti-Toxoplasma gondii immunoglobulins

被引：12

作者：

Alves, Livia M. ^{[1
]}

Banos, Heber L. S. ^{[2
]}

Flauzino, Jose M. R. ^{[1
]}

Guedes, Pedro H. G. ^{[1
]}

Pereira, Janser M. ^{[3
]}

Fujiwara, Ricardo T. ^{[4
]}

Mine, Tiago W. P. ^{[2
]}

Mineo, Jose R. ^{[2
]}

de Oliveira, Ronald J. ^{[5
]}

Madurro, Joao M. ^{[6
]}

Brito-Madurro, Ana G. ^{[1
]}

机构：

[1] Univ Fed Uberlandia, Inst Biotechnol, Uberlandia, MG, Brazil

[2] Univ Fed Uberlandia, Inst Biomed Sci, Uberlandia, MG, Brazil

[3] Univ Fed Uberlandia, Fac Math, Uberlandia, MG, Brazil

[4] Univ Fed Minas Gerais, Inst Biol Sci, Belo Horizonte, MG, Brazil

[5] Univ Fed Triangulo Mineiro, Inst Exact Nat Sci & Educ, Uberaba, Brazil

[6] Univ Fed Uberlandia, Inst Chem, Uberlandia, MG, Brazil

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2019年 / 175卷

关键词：

Toxoplasma gondii; Mimetic peptide; Poly(3-hydroxybenzoic acid); Immunosensor; ELECTROCHEMICAL IMMUNOSENSORS; PROTEIN; BIOSENSOR; ANTIBODIES;

D O I：

10.1016/j.jpba.2019.112778

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Toxoplasma gondii is an intracellular protozoan parasite responsible for toxoplasmosis, which affects humans and animals. Serologic detection of anti-T. gondii immunoglobulins plays a crucial role in the clinical diagnosis of toxoplasmosis. In this work, a novel electrochemical immunosensor for detecting anti-T. gondii immunoglobulins is reported, based on immobilization of an in silico predicted peptide (PepB3), obtained from membrane protein of T. gondii, on the graphite electrode modified with poly(3-hydroxybenzoic acid). Indirect ELISA confirmed infection and binding specificity of peptide PepB3. Molecular modelling and simulations show this peptide binds to the T gondii human Fab antibody in the surface antigen 1 (SAG1) binding site, remaining a stable complex during the molecular dynamic simulations, especially by hydrogen bonds and hydrophobic interactions. This electrochemical immunosensor was able to discriminate different periods of infection, using infected mouse serum samples, showing selectivity and discriminating infected and uninfected mouse serum. (C) 2019 Published by Elsevier B.V.

引用

页数：7

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