Development of SSR markers for studies of diversity in the genus Fagopyrum

被引：65

作者：

Ma, Kyung-Ho ^{[1
]}

Kim, Nam-Soo ^{[2
]}

Lee, Gi-An ^{[1
]}

Lee, Sok-Young ^{[1
]}

Lee, Ju Kyong ^{[3
]}

Yi, Jung Yoon ^{[1
]}

Park, Yong-Jin ^{[4
]}

Kim, Tae-San ^{[1
]}

Gwag, Jae-Gyun ^{[1
]}

Kwon, Soon-Jae ^{[1
]}

机构：

[1] RDA, Natl Acad Agr Sci, Natl Agrobiodivers Ctr, Suwon 441707, Gyunggi Do, South Korea

[2] Kangwon Natl Univ, Coll Biosci & Biotechnol, Chunchon 200701, South Korea

[3] Kangwon Natl Univ, Coll Agr & Life Sci, Chunchon 200701, South Korea

[4] Kongju Natl Univ, Dept Plant Resources, Yesan 340702, South Korea

来源：

THEORETICAL AND APPLIED GENETICS | 2009年 / 119卷 / 07期

关键词：

CULTIVATED COMMON BUCKWHEAT; CROSS-SPECIES AMPLIFICATION; MICROSATELLITE MARKERS; LINKAGE DISEQUILIBRIUM; NUCLEOTIDE-SEQUENCES; WILD ANCESTOR; TRANSFERABILITY; ESCULENTUM; PLANTS; MAIZE;

D O I：

10.1007/s00122-009-1129-8

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The numbers of SSR markers and their utilization have not been determined and investigated as extensively in Fagopyrum species as compared to other crop species. The current report presents 136 new SSR markers in Fagopyrum esculentum ssp. esculentum and their application to related species in the genus Fagopyrum. Of the 136 SSRs, 10 polymorphic SSR markers were utilized in a genetic diversity analysis of a common buckwheat population consisting of 41 accessions of diverse origin. The study showed observed (H (O)) and expected (H (E)) heterozygosities ranging from 0.071 to 0.924 (mean = 0.53) and from 0.073 to 0.902 (mean = 0.412), respectively. Forty-one of the 136 SSRs amplified sequences in other Fagopyrum species, including the cymosum and urophyllum groups. The phylogenetic relationships revealed using the SSRs was consistent with results obtained using other marker systems, with one exception. The sequence and diversity information obtained using these new SSRs and their cross-transferability to related Fagopyrum species will increase our understanding of genetic structures and species relationships within the Fagopyrum genus.

引用

页码：1247 / 1254

页数：8

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