All-polymer-based micro free-flow electrophoresis (μFFE) device with cellulose permeable membranes

被引：3
作者：
Takehara, Hiroaki [1 ,2 ]
Sato, Shusuke [2 ]
Ichiki, Takanori [1 ,2 ]
机构：
[1] Univ Tokyo, Sch Engn, Dept Mat Engn, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan
[2] Innovat Ctr NanoMed iCONM, 3-25-14 Tonomachi, Kawasaki, Kanagawa 2100821, Japan
来源：
APPLIED PHYSICS EXPRESS | 2019年 / 12卷 / 10期
基金：
日本科学技术振兴机构;
关键词：
CAPILLARY-ZONE-ELECTROPHORESIS; SAMPLE PURIFICATION; PROTEIN SEPARATION; FLUORESCENT DYES; DIFFUSION; AFFINITY; CHAMBER; CHIP;
D O I：
10.7567/1882-0786/ab3d64
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
A micro free-flow electrophoresis (mu FFE) device with cellulose membranes as ion-permeable separators was developed. Due to the neutral surface zeta potential of poly(methyl methacrylate-co-styrene), electroosmotic flow was not generated. The developed mu FFE device was evaluated in separations of two fluorescent dyes, rhodamine B and sulforhodamine B. Peak resolution (Rs) at an electric field strength of 11.5 V mm(-1) was 4.1, 2.8, and 1.8 at sample flow velocities of 1.3, 2.0, and 2.7 mm s(-1), respectively. All-polymer-based mu FFE devices are ready for use without pretreatment and satisfy the criteria for practical biological sample separation and purification in biomedical applications. (C) 2019 The Japan Society of Applied Physics.
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页数：4
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