The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood

被引：52

作者：

Cavalcanti, Milena de Paiva ^{[1
]}

Felinto de Brito, Maria Edileuza ^{[1
]}

de Souza, Wayner Vieira ^{[2
]}

Gomes, Yara de Miranda ^{[1
]}

Abath, Frederico G. C. ^{[1
]}

机构：

[1] Fiocruz MS, Ctr Pesquisas Aggeu Magalhaes, Dept Imunol, BR-50670420 Recife, PE, Brazil

[2] Fiocruz MS, Ctr Pesquisas Aggeu Magalhaes, Dept Saude Colet, BR-50670420 Recife, PE, Brazil

来源：

VETERINARY JOURNAL | 2009年 / 182卷 / 02期

关键词：

Visceral leishmaniosis; Leishmania infantum; Real-time PCR (qPCR); Diagnosis; TISSUES;

D O I：

10.1016/j.tvjl.2008.05.018

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Recent research has demonstrated the high sensitivity of real time PCR (qPCR) in the diagnosis of Leishmania infantum infection. The goal of this study was to develop and evaluate a qPCR detection system for the diagnosis of visceral leishmaniosis (VL) in dogs. Specific primer sets were developed for the Leishmania donovani complex, in which a fragment of 132 bp of kDNA from L. infantum was amplified. The reaction was performed using the ABI PRISM 7000 system with ABI PRISM software used to carry out the analysis. When canine blood samples were assessed using this system the detection limit of the method was found to be 0.07 parasites per reaction, the efficiency was 94.17% (R-2 = 0.93, slope = -3.47) and the sensitivity and specificity were 100% and 83.33% respectively. The use of such a sensitive, reproducible and rapid qPCR-based assay will be useful in the diagnosis and control of L. infantum infection in endemic areas, where serological surveys often underestimate true disease prevalence. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：356 / 358

页数：3

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