Differential brain-derived neurotrophic factor and transforming growth factor-beta expression in the rat cochlea following deafness

被引：16
作者：
Wissel, Kirsten
Wefstaedt, Patrick
Miller, Josef M.
Lenarz, Thomas
Stoever, Timo
机构：
[1] Univ Michigan, Kresge Hearing Res Inst, Ann Arbor, MI 48109 USA
[2] Univ Hannover, Dept Otolaryngol, D-30625 Hannover, Germany
来源：
NEUROREPORT | 2006年 / 17卷 / 12期
关键词：
auditory nerve; brain-derived neurotrophic factor; immunohistochemistry; inferior colliculus; neomycin-induced deafness; semi-quantitative reverse-transcriptase polymerase chain reaction; transforming growth factor family;
D O I：
10.1097/01.wnr.0000233088.92839.23
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Brain derived neurotrophic factor (BDNF) and transforming growth factor-beta (TGF beta) subtypes have demonstrated their importance in cochlear functions. The aim of this study was to determine gene and protein expression patterns of BDNF, TGF beta 1/2 and their main receptors trkB and TGF beta R1/R2 in the auditory nerve and inferior colliculus of normal hearing and deafened rats by reverse-transcriptase polymerase chain reaction and immunohistochemistry. Deafening was performed by cochlear injection of neomycin (10%). Significant gene expression changes were not found in the inferior colliculus after deafness; however, in the auditory nerve, BDNF, TGF beta 1 and TGF beta R1 mRNA in particular were upregulated. Additionally, BDNF protein and the cytokines were found to be expressed in the auditory nerve after hair cell loss. These data indicate the importance of BDNF and TGF beta 1 as endogenous survival factors.
引用
收藏
页码：1297 / 1301
页数：5
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