Core/Shell Polymer Particles by Semibatch Combined Suspension/Emulsion Polymerizations for Enzyme Immobilization

被引:29
作者
Besteti, Marina D. [1 ]
Cunha, Aline G. [2 ]
Freire, Denise M. G. [2 ]
Pinto, Jose Carlos [1 ]
机构
[1] Univ Fed Rio de Janeiro, COPPE, Programa Engn Quim, BR-21941972 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Programa Posgrad Bioquim, Inst Quim, Ctr Tecnol, BR-21941909 Rio De Janeiro, RJ, Brazil
关键词
core-shell polymers; emulsion polymerization; enzymes; immobilization; suspension polymerization; LIPASE;
D O I
10.1002/mame.201300023
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Semibatch combined suspension-emulsion polymerizations are performed for production of polymer supports to be used for immobilization of lipase B from Candida antarctica (CALB), using styrene, methyl methacrylate, and cardanol as monomers. It is shown that the obtained polymer particles present the characteristic core-shell particle structure, with specific areas and average pore sizes that are comparable to values presented by Accurel MP 1000. It is also shown that the produced particles can be used successfully for immobilization of CALB, leading to immobilization efficiencies and enzyme activities that are better than the ones obtained with Accurel MP 1000.
引用
收藏
页码:135 / 143
页数:9
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