Glycerol and neutral lipid production in the oleaginous marine diatom Phaeodactylum tricornutum promoted by overexpression of glycerol-3-phosphate dehydrogenase

被引：99

作者：

Yao, Yao ^{[1
]}

Lu, Yang ^{[1
]}

Peng, Kun-Tao ^{[1
]}

Huang, Tan ^{[1
]}

Niu, Ying-Fang ^{[1
]}

Xie, Wei-Hong ^{[1
]}

Yang, Wei-Dong ^{[1
]}

Liu, Jie-Sheng ^{[1
]}

Li, Hong-Ye ^{[1
]}

机构：

[1] Jinan Univ, Coll Life Sci, Guangdong Higher Educ Inst, Key Lab Eutrophicat & Red Tide Prevent, Guangzhou 510632, Guangdong, Peoples R China

来源：

BIOTECHNOLOGY FOR BIOFUELS | 2014年 / 7卷

关键词：

Glycerol-3-phosphate dehydrogenase; Glycerol; Diatom; Lipid; SACCHAROMYCES-CEREVISIAE; CHLAMYDOMONAS-REINHARDTII; CLONING; ACCUMULATION; EXPRESSION; ORGANIZATION; MICROALGAE; BIODIESEL; GROWTH; MODEL;

D O I：

10.1186/1754-6834-7-110

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Microalgae are ideal raw materials for biodiesel and bioactive compounds. Glycerol-3-phosphate is formed from dihydroxyacetone phosphate (DHAP) through the glycolytic pathway catalyzed by glycerol-3-phosphate dehydrogenase (GPDH). Results: GPDH was characterized in the marine diatom Phaeodactylum tricornutum. In the GPDH-overexpressing P. tricornutum cells, the glycerol concentration per cell in the transformed diatom increased by 6.8-fold compared with the wild type, indicating that the overexpression of GPDH promoted the conversion of DHAP to glycerol-3-phosphate. There was a 60% increase in neutral lipid content, reaching 39.7% of dry cell weight in transgenic cells in the stationary phase, despite a 20% decrease in cell concentration. Fatty acid profiling showed that the levels of 16-and 18-carbon monounsaturated fatty acids significantly increased. Conclusion: GPDH had a significant impact on numerous metabolic processes in diatom cells, including the biosynthesis of glycerol and neutral lipids. These findings are instructive for the metabolic engineering of microalgae for biofuel production.

引用

页数：9

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