Synthesis of dinitrophenyl-L-Pro-N-hydroxysuccinimide ester and four new variants of Sanger's reagent having chiral amines and their application for enantioresolution of mexiletine using reversed-phase high-performance liquid chromatography

被引：22

作者：

Bhushan, Ravi ^{[1
]}

Tanwar, Shivani ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Roorkee 247667, Uttar Pradesh, India

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 30期

关键词：

Mexiletine; Indirect chiral separation; RP-HPLC; Marfey's reagent; Dinitrophenyl-L-Pro-N-hydroxysuccinimide ester; MARFEYS REAGENT; STRUCTURAL VARIANTS; INDIRECT RESOLUTION; ENANTIOMERS; SEPARATION; PLASMA; ACIDS; PENICILLAMINE; ALCOHOLS; ASSAY;

D O I：

10.1016/j.chroma.2009.05.086

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four chiral derivatizing reagents (CDRs) having enantiomerically pure amines and two CDRs namely. [N-succinimidyl-(S)-2-(6-methoxynaphth-2-yl)propionate], and dinitrophenyl-L-Pro-N-hydroxysuccinimide ester, DNP-L-Pro-SU] were synthesized and were used to prepare diastereomers of (R,S)-mexiletine (MEX); these were separated by reversed-phase high-performance liquid chromatography (RP-HPLC). The method was validated for linearity, accuracy, limit of detection (LOD) and limit of quantification (LOQ). (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：5769 / 5773

页数：5

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