Fluorescence Detection of DNA Hybridization Based on the Aggregation-Induced Emission of a Perylene-Functionalized Polymer

被引：35
作者：
Wang, Guojie [1 ]
Zhang, Ruichen [1 ]
Xu, Chen [1 ]
Zhou, Ruyi [1 ]
Dong, Jie [1 ]
Bai, Huitao [2 ]
Zhan, Xiaowei [2 ]
机构：
[1] Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
[2] Peking Univ, Dept Mat Sci & Engn, Coll Engn, Beijing 100871, Peoples R China
来源：
ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 14期
基金：
中国国家自然科学基金;
关键词：
aggregation-induced emission; fluorescence; hybridization; polyelectrolyte; probe; TURN-ON DETECTION; PYRENE; MULTICOLOR; COMPLEXES; AMMONIUM; BINDING; PROBES; BEACON; ASSAY; RNA;
D O I：
10.1021/am503171s
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
A perylene-functionalized polycation was synthesized by quaternization of poly(4-vinylpyridine) with bromomethyl-perylene and methyl iodide, which exhibited a unique aggregation-induced emission (AIE) effect. The synthesized polycation and polyanion ssDNA could form a complex probe to detect DNA hybridization. Upon adding noncomplementary ssDNA, the fluorescence of the complex probe increased due to the AIE effect; upon adding complementary ssDNA, the fluorescence intensity changed little due to the combined effects of AIE and duplex-quenching resulting from the intercalation of perylene into the duplex.
引用
收藏
页码：11136 / 11141
页数：6
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