Solid-phase synthesis of branched RNA and branched DNA/RNA chimeras

被引：27
|
作者：
Grotli, M [1 ]
Eritja, R [1 ]
Sproat, B [1 ]
机构：
[1] INNOVIR, D-37124 ROSDORF, GERMANY
来源：
TETRAHEDRON | 1997年 / 53卷 / 33期
关键词：
D O I：
10.1016/S0040-4020(97)00731-X
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
An effective method for synthesising branched oligonucleotides on solid phase in the 5' to 3' direction has been developed. Special branch-point monomers enable the synthesis of branched oligonucleotides which can have sequences of different length and base composition attached to the 2'-and 3'-hydroxyl groups of the branch point ribonucleoside. The branched oligonucleotides are assembled on commercial DNA synthesisers, the crude products are readily purified by reversed phase HPLC and the fully deprotected products are conveniently analysed by mass spectrometry. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：11317 / 11346
页数：30
相关论文
共 50 条
  • [1] Solid-phase Synthesis of Branched RNA and Branched DNA/RNA Chimeras
    Groetli, M.
    Eritja, R.
    Sproat, B.
    Tetrahedron, 53 (33):
  • [2] NOVEL SOLID-PHASE SYNTHESIS OF BRANCHED OLIGORIBONUCLEOTIDES, INCLUDING A SUBSTRATE FOR THE RNA DEBRANCHING ENZYME
    SPROAT, BS
    BEIJER, B
    GROTLI, M
    RYDER, U
    MORAND, KL
    LAMOND, AI
    JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1994, (04): : 419 - 431
  • [3] Regiospecific Solid-Phase Synthesis of Branched Oligoribonucleotides That Mimic Intronic Lariat RNA Intermediates
    Katolik, Adam
    Johnsson, Richard
    Montemayor, Eric
    Lackey, Jeremy G.
    Hart, P. John
    Damha, Masad J.
    JOURNAL OF ORGANIC CHEMISTRY, 2014, 79 (03): : 963 - 975
  • [4] SOLID-PHASE SYNTHESIS OF BRANCHED OLIGORIBONUCLEOTIDES RELATED TO MESSENGER-RNA SPLICING INTERMEDIATES
    DAMHA, MJ
    GANESHAN, K
    HUDSON, RHE
    ZABARYLO, SV
    NUCLEIC ACIDS RESEARCH, 1992, 20 (24) : 6565 - 6573
  • [5] Solid-phase synthesis of branched thiohydantoin benzimidazolinethiones and branched thiohydantoin tetrahydroquinoxalinediones
    Nefzi, A
    Giulianotti, MA
    Houghten, RA
    TETRAHEDRON LETTERS, 2000, 41 (14) : 2283 - 2287
  • [6] AUTOMATED SOLID-PHASE SYNTHESIS OF BRANCHED OLIGONUCLEOTIDES
    DAMHA, MJ
    ZABARYLO, S
    TETRAHEDRON LETTERS, 1989, 30 (46) : 6295 - 6298
  • [7] A UNIVERSAL SOLID-PHASE SYNTHESIS OF BRANCHED OLIGORIBONUCLEOTIDES
    GROTLI, M
    SPROAT, BS
    JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1995, (04) : 495 - 497
  • [8] Improved deoxyribozymes for synthesis of covalently branched DNA and RNA
    Lee, Christine S.
    Mui, Timothy P.
    Silverman, Scott K.
    NUCLEIC ACIDS RESEARCH, 2011, 39 (01) : 269 - 279
  • [9] BRANCHED RNA
    EDMONDS, M
    BIOESSAYS, 1987, 6 (05) : 212 - 216
  • [10] Solid-phase synthesis of transition metal linked, branched oligonucleotides
    Vargas-Baca, I
    Mitra, D
    Zulyniak, HJ
    Banerjee, J
    Sleiman, HF
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2001, 40 (24) : 4629 - +
12345