Recovery of virulent and RNase-negative attenuated type 2 bovine viral diarrhea viruses from infectious cDNA clones

被引：52
作者：
Meyer, C
von Freyburg, M
Elbers, K
Meyers, G
机构：
[1] Fed Res Ctr Virus Dis Anim, Inst Immunol, D-72076 Tubingen, Germany
[2] Boehringer Ingelheim Vetmed GmbH, D-52216 Ingelheim, Germany
来源：
JOURNAL OF VIROLOGY | 2002年 / 76卷 / 16期
关键词：
D O I：
10.1128/JVI.76.16.8494-8503.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Cloned cDNA derived from the genome of the virulent type 2 bovine viral diarrhea virus (BVDV) strain NY'93/C was sequenced and served for establishment of the infectious cDNA clone pKANE40A. Virus recovered from pKANE40A exhibited growth characteristics similar to those of wild-type BVDV NY'93/C and proved to be clinically indistinguishable from the wild-type virus in animal experiments. A virus mutant in which the RNase residing in the viral glycoprotein E-rns Was inactivated, revealed an attenuated phenotype. The plasmid pKANE40A represents the first infectious cDNA clone established for a type 2 BVDV and offers a variety of new approaches to analyze the mechanisms of BVDV-induced disease in cattle.
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页码：8494 / 8503
页数：10
相关论文
共 67 条
[1]  
[Anonymous], 1989, MOL CLONING LAB MANU
[2]  
BAKER JC, 1987, J AM VET MED ASSOC, V190, P1449
[3]   MOLECULAR CHARACTERIZATION OF BORDER DISEASE VIRUS, A PESTIVIRUS FROM SHEEP [J].
BECHER, P ;
SHANNON, AD ;
TAUTZ, N ;
THIEL, HJ .
VIROLOGY, 1994, 198 (02) :542-551
[4]   Genetic diversity of pestiviruses:: Identification of novel groups and implications for classification [J].
Becher, P ;
Orlich, M ;
Kosmidou, A ;
König, M ;
Baroth, M ;
Thiel, HJ .
VIROLOGY, 1999, 262 (01) :64-71
[5]  
BOLIN SR, 1992, AM J VET RES, V53, P2157
[6]  
BOLIN SR, 1991, AM J VET RES, V52, P1033
[7]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE PESTIVIRUS BOVINE VIRAL DIARRHEA VIRUS [J].
COLLETT, MS ;
LARSON, R ;
GOLD, C ;
STRICK, D ;
ANDERSON, DK ;
PURCHIO, AF .
VIROLOGY, 1988, 165 (01) :191-199
[8]   PROTEINS ENCODED BY BOVINE VIRAL DIARRHEA VIRUS - THE GENOMIC ORGANIZATION OF A PESTIVIRUS [J].
COLLETT, MS ;
LARSON, R ;
BELZER, SK ;
RETZEL, E .
VIROLOGY, 1988, 165 (01) :200-208
[9]  
COLLETT MS, 1991, ARCH VIROL, P19
[10]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF A PESTIVIRUS GENOME, NONCYTOPATHIC BOVINE VIRAL DIARRHEA VIRUS STRAIN-SD-1 [J].
DENG, RT ;
BROCK, KV .
VIROLOGY, 1992, 191 (02) :867-879
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