TGF-β1 Upregulates the Expression of Triggering Receptor Expressed on Myeloid Cells 1 in Murine Lungs

被引：13
作者：
Peng, Li [1 ]
Zhou, Yong [1 ]
Dong, Liang [1 ]
Chen, Rui-Qi [1 ]
Sun, Guo-Ying [1 ]
Liu, Tian [1 ]
Ran, Wen-Zhuo [1 ]
Fang, Xiang [2 ]
Jiang, Jian-Xin [3 ]
Guan, Cha-Xiang [1 ]
机构：
[1] Cent South Univ, Xiangya Sch Med, Dept Physiol, Changsha, Hunan, Peoples R China
[2] Univ Texas Med Branch, Dept Neurol, Galveston, TX 77555 USA
[3] Third Mil Med Univ, Daping Hosp, Inst Surg Res, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词：
PULMONARY-FIBROSIS; PERIINFARCT TISSUE; C-JUN; TREM-1; INFLAMMATION; METHYLATION; MACROPHAGES; NEUTROPHILS; ACTIVATION; RESPONSES;
D O I：
10.1038/srep18946
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Triggering receptor expressed on myeloid cells 1 (TREM-1) increases the expression of TGF-beta family genes, which are known as profibrogenic cytokines in the pathogenesis of pulmonary fibrosis. In this study, we determined whether TGF-beta 1 regulated the expression of TREM-1 in a mouse model of pulmonary fibrosis. The expression of TGF-beta 1 and TREM-1 was increased on day 7, 14, and 21 after single intratracheal injection of bleomycin (BLM). And there was positive correlation between the expression of TGF-beta 1 and TREM-1. TGF-beta 1 increased expression of TREM-1 mRNA and protein in a time-and dose-dependent manner in mouse macrophages. The expression of the activator protein 1 (AP-1) was increased in lung tissues from mouse after BLM injection and in mouse macrophages after TGF-beta 1 treatment, respectively. TGF-beta 1 significantly increased the relative activity of luciferase in the cells transfected with plasmid contenting wild type-promoter of TREM-1. But TGF-beta 1 had no effect on the activity of luciferase in the cells transfected with a mutant-TREM1 plasmid carrying mutations in the AP-1 promoter binding site. In conclusion, we found the expression of TREM-1 was increased in lung tissues from mice with pulmonary fibrosis. TGF-beta 1 increased the expression of TREM-1 in mouse macrophages partly via the transcription factor AP-1.
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页数：9
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