Detection of white spot syndrome virus in filtered shrimp-farm water fractions and experimental evaluation of its infectivity in Penaeus (Litopenaeus) vannamei

被引:34
作者
Esparza-Leal, Hector M. [2 ,3 ]
Escobedo-Bonilla, Cesar M. [4 ]
Casillas-Hernandez, Ramon [3 ]
Alvarez-Ruiz, Pindaro [2 ]
Portillo-Clark, Guillermo [1 ]
Valerio-Garcia, Roberto C. [4 ]
Hernandez-Lopez, Jorge [4 ]
Mendez-Lozano, Jesus [2 ]
Vibanco-Perez, Norberto [5 ]
Magallon-Barajas, Francisco J. [1 ]
机构
[1] Ctr Invest Biol Noroeste, La Paz 23090, Baja Calif Sur, Mexico
[2] Ctr Interdisciplinario Invest Desarrollo Integral, Unidad Sinaloa, Guasave 81101, Sinaloa, Mexico
[3] Inst Tecnol Sonora, Programa Doctorado Ciencias Biotecnol, Obregon 85000, Sonora, Mexico
[4] Ctr Invest Biol Noroeste, Unidad Sonora, Hermosillo 83260, Sonora, Mexico
[5] Univ Autonoma Nayarit, Tepic 63190, Nayarit, Mexico
关键词
Penaeus (Litopenaeus) vannamei; WSSV; Shrimp farm; Differential filtration; Pond water; Infectivity assay; WSSV TRANSMISSION; TEMPERATURE; 33-DEGREES-C; NESTED PCR; BACULOVIRUS; MONODON; JAPONICUS; VECTOR; MEXICO; CRABS; INDIA;
D O I
10.1016/j.aquaculture.2009.03.021
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
White spot syndrome virus (WSSV) may spread through water to neighbor ponds or farms. Routine water exchange and wastewater released during white spot disease (WSD)-emergency harvests may preserve WSSV in shrimp farming areas. To test this hypothesis, on-site experiments were performed in a WSSV-affected farm in Guasave, Sinaloa, Mexico. Plankton and shrimp hemolymph were collected from 12 ponds during a WSD outbreak. PCR analyses showed that 72% of the hemolymph pools (26 out of 36) were WSSV-positive. In contrast, only 14% (4 of 28) plankton samples (filtered through 10 and 0.45 mu m) from three ponds (2, 7 and 10) were WSSV-positive. Plankton from pond 9 was WSSV-negative, but 14 days later, shrimp began to die. At this point, a differential filtration experiment was performed in pond 9. WSSV-positive samples were only found in three fractions [particulate fraction (PF) 1 mu m and liquid fractions (LF) <100 and <40 mu m]. Both LFs and PFs were used for in situ infectivity assays by water-borne routes in WSSV-negative whiteleg shrimp Penaeus (Litopenaeus) vannamei. Some shrimp exposed to different PFs and LFs (100 mu m to >0.65 mu m) became WSSV-positive. Results indicate that water fractions between 100 and 0.65 mu m induced WSSV infection to shrimp. Results showed that pond water and/or particulate fractions are vehicles for WSSV dispersion via virus suspended in water, attached to microalgae, or carried by zooplankton. (C) 2009 Published by Elsevier B.V.
引用
收藏
页码:16 / 22
页数:7
相关论文
共 46 条
[1]  
Chakraborty A, 2002, CURR SCI INDIA, V82, P1392
[2]   Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan [J].
Chou, HY ;
Huang, CY ;
Wang, CH ;
Chiang, HC ;
Lo, CF .
DISEASES OF AQUATIC ORGANISMS, 1995, 23 (03) :165-173
[3]  
CLIFFORD HC, 1999, AQUACULTURE 99, P134
[4]   Characterization of water quality factors during intensive raceway production of juvenile Litopenaeus vannamei using limited discharge and biosecure management tools [J].
Cohen, JM ;
Samocha, TM ;
Fox, JM ;
Gandy, RL ;
Lawrence, AL .
AQUACULTURAL ENGINEERING, 2005, 32 (3-4) :425-442
[5]  
Corsin F., 2005, Diseases in Asian Aquaculture V, Fish Health Section, P75
[6]  
de la Rosa-Vélez Jorge, 2006, Hidrobiológica, V16, P275
[7]  
Escobedo-Bonilla CM, 2008, J FISH DIS, V31, P1, DOI 10.1111/j.1365-2761.2007.00877.x
[8]   In vivo titration of white spot syndrome virus (WSSV) in specific pathogen-free Litopenaeus vannamei by intramuscular and oral routes [J].
Escobedo-Bonilla, CM ;
Wille, M ;
Sanz, VA ;
Sorgeloos, P ;
Pensaert, MB ;
Nauwynck, HJ .
DISEASES OF AQUATIC ORGANISMS, 2005, 66 (02) :163-170
[9]   Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand [J].
Flegel, T. W. .
AQUACULTURE, 2006, 258 (1-4) :1-33
[10]   The crisis in Asian shrimp aquaculture: current status and future needs [J].
Flegel, TW ;
Alday-Sanz, V .
JOURNAL OF APPLIED ICHTHYOLOGY-ZEITSCHRIFT FUR ANGEWANDTE ICHTHYOLOGIE, 1998, 14 (3-4) :269-273