High-throughput, multiparameter analysis of single cells

被引：36
作者：
Haselgruebler, Thomas [1 ]
Haider, Michaela [1 ]
Ji, Bozhi [1 ]
Juhasz, Kata [1 ]
Sonnleitner, Alois [1 ]
Balogi, Zsolt [1 ]
Hesse, Jan [1 ]
机构：
[1] Ctr Adv Bioanal GmbH, A-4020 Linz, Austria
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2014年 / 406卷 / 14期
关键词：
Single-cell analysis; Population heterogeneity; Microfluidics; High-throughput screening; Genomics/proteomics; Nucleic acids (DNA/RNA); CIRCULATING TUMOR-CELLS; GENE-EXPRESSION; FLOW-CYTOMETRY; STEM-CELLS; RNA-SEQ; FLUORESCENCE MICROSCOPY; PERSONALIZED MEDICINE; TARGET-ENRICHMENT; CANCER-RESEARCH; MASS CYTOMETRY;
D O I：
10.1007/s00216-013-7485-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Heterogeneity of cell populations in various biological systems has been widely recognized, and the highly heterogeneous nature of cancer cells has been emerging with clinical relevance. Single-cell analysis using a combination of high-throughput and multiparameter approaches is capable of reflecting cell-to-cell variability, and at the same time of unraveling the complexity and interdependence of cellular processes in the individual cells of a heterogeneous population. In this review, analytical methods and microfluidic tools commonly used for high-throughput, multiparameter single-cell analysis of DNA, RNA, and proteins are discussed. Applications and limitations of currently available technologies for cancer research and diagnostics are reviewed in the light of the ultimate goal to establish clinically applicable assays.
引用
收藏
页码：3279 / 3296
页数：18
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