Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes

被引：18

作者：

Fujimoto, Takuya ^{[1
]}

Tsunedomi, Ryouichi ^{[1
]}

Matsukuma, Satoshi ^{[1
]}

Yoshimura, Kiyoshi ^{[2
]}

Oga, Atsunori ^{[3
]}

Fujiwara, Nobuyuki ^{[1
]}

Fujiwara, Yasuhiro ^{[1
]}

Matsui, Hiroto ^{[1
]}

Shindo, Yoshitaro ^{[1
]}

Tokumitsu, Yukio ^{[1
]}

Suzuki, Nobuaki ^{[1
]}

Kobayashi, Shogo ^{[4
]}

Hazama, Shoichi ^{[5
]}

Eguchi, Hidetoshi ^{[4
]}

Nagano, Hiroaki ^{[1
]}

机构：

[1] Yamaguchi Univ, Dept Gastroenterol Breast & Endocrine Surg, Grad Sch Med, 1-1-1 Minami Kogushi, Ube, Yamaguchi 7558505, Japan

[2] Showa Univ, Dept Clin Res Tumor Immunol, Clin Res Inst Clin Pharmacol & Therapeut, Setagaya Ku, Tokyo 1578577, Japan

[3] Yamaguchi Univ, Dept Mol Pathol, Grad Sch Med, Ube, Yamaguchi 7558505, Japan

[4] Osaka Univ, Dept Gastroenterol Surg, Grad Sch Med, Suita, Osaka 5650871, Japan

[5] Yamaguchi Univ, Dept Translat Res & Dev Therapeut Canc, Sch Med, Ube, Yamaguchi 7558505, Japan

来源：

ONCOLOGY LETTERS | 2021年 / 21卷 / 01期

关键词：

pancreatic cancer; cancer stem cells; biomarker; cathepsin B; GASTRIC-CANCER; TUMOR-GROWTH; INVASION; OVEREXPRESSION; TRANSCRIPTION; ACTIVATION; PROTEOMICS; PROTEASES; FEATURES; ROLES;

D O I：

10.3892/ol.2020.12291

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cancer stem-like cells (CSLCs) in solid tumors are resistant to conventional chemotherapy and molecularly targeted therapy, which is thought to contribute to cancer recurrence and metastasis. The present study aimed to identify biomarkers for pancreatic CSLCs (P-CSLCs). Using our previously reported methods, P-CSLC-enriched populations were generated from pancreatic cancer cell lines. The protein expression profiles of these populations were compared with those of parental cells using two-dimensional electrophoresis, tandem mass spectrometry, flow cytometry and immunohistochemistry. Protein expression in surgical specimens was also evaluated for relationships with clinical outcomes. A lysosomal cysteine protease, cathepsin B (CTSB), was significantly upregulated in P-CSLCs compared with that in the parental cells, as shown using western blotting. Flow cytometry analysis also confirmed that CTSB was more highly expressed on the surface of P-CSLCs compared with that on parental cells. Moreover, PCLCs had elevated cellular secretions of CTSB compared with the parental cells. Finally, CTSB expression was evaluated in 69 resected tumor specimens, and high expression was associated with the patients' clinicopathological features and surgical outcomes. The present results suggested that CTSB is a biomarker for poor survival in patients with pancreatic cancer, which is possibly associated with P-CSLCs. This novel biomarker may also have potential as a therapeutic target.

引用

页码：1 / 9

页数：9

共 51 条

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