Analysis of organoselenium and organic acid metabolites by laser desorption single photon ionization mass spectrometry

被引：22

作者：

Chen, Yanfeng ^{[1
]}

Sullards, M. Cameron ^{[1
]}

Hoang, Tiffany T. ^{[1
]}

May, Sheldon W. ^{[1
]}

Orlando, Thomas M. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 24期

关键词：

D O I：

10.1021/ac060827x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for analyzing organoselenium and organic acid metabolites using laser desorption from graphite surfaces coupled to vacuum ultraviolet single photon ionization mass spectrometry (LD/SPI MS) is described. The 1- 10-fmol sensitivity and linear dynamic range allows quantitative detection of selenomethionine, trimethylselenonium ion, methylselenogalactosamine, and 1 beta-methylseleno-N-acetyl-D- galactosamine in complex biological samples such as human urine. In addition, common urinary metabolites such as tartronic, glutaric, orotic, uric, suberic, and hydroxyhippuric acids, are readily detected. Screening and quantitative detection of these organoselenium and organic acid metabolites is achieved within minutes. The results are also consistent with those obtained using high-performance liquid chromatography tandem mass spectrometry techniques. The study demonstrates the viability of matrix-free LD/SPI MS for molecular characterization and quantitive analysis of biological metabolites in the m/z 10-500 range that are present in complex biological fluids.

引用

页码：8386 / 8394

页数：9

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