Enantioseparation by MEKC using a ligand exchange-based chiral pseudostationary phase

被引：11

作者：

Zaher, Mustapha ^{[1
]}

Ravelet, Corinne ^{[1
]}

Vanhaverbeke, Cecile ^{[1
]}

Baussanne, Isabelle ^{[1
]}

Perrier, Sandrine ^{[1
]}

Fize, Jennifer ^{[1
]}

Decout, Jean-Luc ^{[1
]}

Peyrin, Eric ^{[1
]}

机构：

[1] Univ Grenoble 1, Inst Chim Mol Grenoble FR 2607, CNRS, Dept Pharmacochim Mol,UFR Pharm,UMR 5063, F-38041 Grenoble 9, France

来源：

ELECTROPHORESIS | 2009年 / 30卷 / 16期

关键词：

Ligand-exchange; MEKC; Neamine derivative; ELECTROKINETIC CAPILLARY CHROMATOGRAPHY; UNDERIVATIZED AMINO-ACIDS; ENANTIOMERIC SEPARATION; TERNARY COMPLEX; SELECTOR; DERIVATIVES; CE;

D O I：

10.1002/elps.200800832

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, a new ligand-exchange -MEKC mode, based on the design of a unique lipohilic species (4'-octadecylneamine derivative), which served both as micelle-forming surfactant (by its hydrophobic part) and central ion-complexing ligand (by its hydrophilic part) is described. The CMC of the used lipophilic neamine derivative was first determined by surface tension measurements. Subsequent NMR experiments were performed in order to investigate the Cu(II) binding properties of the neamine micellar phase. The enantioseparation proper-ties of both the octadecylneamine derivative-Cu(II) MEKC and the native neamine-Cu(II) CE systems were evaluated and compared using the tryptophan racemate as a probe analyte. The effects of several different electrophoretic conditions on the enantiomer migration behavior in the ligand-exchange-MEKC mode were examined. The developed methodology was also applied to the enantioseparation of other analytes such as 1-methyl-tryptophan, 3,5-diiodo-tyrosine and 1-naphtyl-alanine.

引用

页码：2869 / 2873

页数：5

共 21 条

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