Evaluation of GenoType Mycobacteria Direct Assay in Comparison with Gen-Probe Mycobacterium tuberculosis Amplified Direct Test and GenoType MTBDRplus for Direct Detection of Mycobacterium tuberculosis Complex in Clinical Samples

被引:15
作者
Neonakis, Ioannis K. [1 ]
Gitti, Zoe [1 ]
Baritaki, Stavroula [2 ]
Petinaki, Efi [3 ]
Baritaki, Maria [1 ]
Spandidos, Demetrios A. [1 ,2 ]
机构
[1] Univ Hosp Heraklion, Dept Clin Bacteriol Parasitol Zoonoses & Geog Med, Mycobacteriol Lab, Iraklion 71201, Crete, Greece
[2] Univ Crete, Sch Med, Dept Lab Med, Iraklion, Greece
[3] Univ Thessaly, Sch Med, Dept Microbiol, Larisa, Greece
关键词
EXTRAPULMONARY SPECIMENS; RIFAMPIN;
D O I
10.1128/JCM.02351-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplified Direct Test, 89.6%. The specificity of the assays used was 100%.
引用
收藏
页码:2601 / 2603
页数:3
相关论文
共 8 条
[1]   Evaluation of the GenoType mycobacteria direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples [J].
de Luna, F. Franco-Alvarez ;
Ruiz, P. ;
Gutierrez, J. ;
Casal, M. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :3025-3027
[2]   Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens [J].
Hillemann, Doris ;
Ruesch-Gerdes, Sabine ;
Richter, Elvira .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) :2635-2640
[3]  
Koneman EW., 1997, Color atlas and Textbook of Diagnostic microbiology, V5th, P893
[4]   Molecular diagnostic tools in mycobacteriology [J].
Neonakis, Ioannis K. ;
Gitti, Zoe ;
Krambovitis, Elias ;
Spandidos, Demetrios A. .
JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 75 (01) :1-11
[5]   Performance assessment of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex from respiratory and extrapulmonary specimens [J].
Piersimoni, C ;
Scarparo, C ;
Piccoli, P ;
Rigon, A ;
Ruggiero, G ;
Nista, D ;
Bornigia, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4138-4142
[6]   Comparative evaluation of the new gen-probe Mycobacterium tuberculosis amplified direct test and the semiautomated Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens [J].
Piersimoni, C ;
Callegaro, A ;
Scarparo, C ;
Penati, V ;
Nista, D ;
Bornigia, S ;
Lacchini, C ;
Scagnelli, M ;
Santini, G ;
De Sio, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (12) :3601-3604
[7]   Comparison of enhanced Mycobacterium tuberculosis amplified direct test with COBAS AMPLICOR Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens [J].
Scarparo, C ;
Piccoli, P ;
Rigon, A ;
Ruggiero, G ;
Scagnelli, M ;
Piersimoni, C .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (04) :1559-1562
[8]   Use of smear-positive samples to assess the PCR-based genotype MTBDR assay for rapid, direct detection of the Mycobacterium tuberculosis complex as well as its resistance to isoniazid and rifampin [J].
Somoskovi, Akos ;
Dormandy, Jillian ;
Mitsani, Dimitra ;
Rivenburg, Jeremy ;
Salfinger, Max .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (12) :4459-4463