Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency

被引:19
|
作者
Zheng, Ye [1 ]
He, Xiao-Wei [1 ]
Ying, Ying-Hui [1 ]
Lu, Jiang-Feng [1 ]
Gelvin, Stanton B. [2 ]
Shou, Hui-Xia [1 ]
机构
[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China
[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
基金
美国国家科学基金会; 中国国家自然科学基金;
关键词
Arabidopsis; histone H2A; Agrobacterium; rice; transformation; CONSTITUTIVE EXPRESSION; PLANT TRANSFORMATION; AGROBACTERIUM; PROTEIN; SUSCEPTIBILITY; MUTANT;
D O I
10.1093/mp/ssp038
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.
引用
收藏
页码:832 / 837
页数:6
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