Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency

被引：19

作者：

Zheng, Ye ^{[1
]}

He, Xiao-Wei ^{[1
]}

Ying, Ying-Hui ^{[1
]}

Lu, Jiang-Feng ^{[1
]}

Gelvin, Stanton B. ^{[2
]}

Shou, Hui-Xia ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China

[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

来源：

MOLECULAR PLANT | 2009年 / 2卷 / 04期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

Arabidopsis; histone H2A; Agrobacterium; rice; transformation; CONSTITUTIVE EXPRESSION; PLANT TRANSFORMATION; AGROBACTERIUM; PROTEIN; SUSCEPTIBILITY; MUTANT;

D O I：

10.1093/mp/ssp038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.

引用

页码：832 / 837

页数：6

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