Metabolomic profiling of rat serum associated with isoproterenol-induced myocardial infarction using ultra-performance liquid chromatography/time-of-flight mass spectrometry and multivariate analysis

被引：64
作者：
Zhang, Hong-yang [1 ,2 ]
Chen, Xi [2 ]
Hu, Ping [1 ]
Liang, Qiong-lin [2 ]
Liang, Xiao-ping [1 ,2 ]
Wang, Yi-ming [2 ]
Luo, Guo-an [1 ,2 ]
机构：
[1] E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
[2] Tsinghua Univ, Key Lab Biorgan Phosphorus Chem & Chem Biol, Dept Chem, Beijing 100084, Peoples R China
来源：
TALANTA | 2009年 / 79卷 / 02期
关键词：
Metabolomics; Ultra-performance liquid chromatography/time-of-flight mass; spectrometry (UPLC/TOF-MS); Multivariate analysis; Myocardial infarction; Lipid biomarkers; LIPID-PEROXIDATION; ARACHIDONIC-ACID; HEART-DISEASE; METABONOMICS; REPERFUSION; ISCHEMIA; URINE; MS;
D O I：
10.1016/j.talanta.2009.03.045
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOF-MS)-based metabolomic approach was developed to characterize the metabolic profile associated with isoproterenol (ISO)-induced myocardial infarction (MI). Analysis of the serum samples revealed distinct changes in the biochemical patterns of ISO-induced rats. A multivariate statistical method, supervised partial least squares-discriminant analysis (PLS-DA), was then used for screening of potential biomarkers. As a result, 13 lipid biomarkers, including lysophosphatidylcholines (Lyso-PCs) and fatty acids were identified by the accurate mass measurement of TOF-MS. The relationship between abnormal lipid metabolism and the formation of MI were also studied. This work demonstrates the utility of UPLC/TOF-MS-based metabolic profiling combined with multivariate analysis as a powerful tool to further investigate the pathogenesis of cardiovascular diseases. (C) 2009 Elsevier B.V. All rights reserved.
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收藏
页码：254 / 259
页数：6
相关论文
共 24 条
  • [1] EFFECT OF ISOPROTERENOL ON THE METABOLISM OF MYOCARDIAL FATTY-ACIDS
    ALMAKDESSI, S
    ANDRIEU, JL
    HERILIER, H
    FAUCON, G
    [J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1987, 19 (02) : 141 - 149
  • [2] Effect of isoproterenol on lipid peroxidation and antioxidant enzymes of myocardial tissue of mice and protection by quinidine
    Chattopadhyay, A
    Biswas, S
    Bandyopadhyay, D
    Sarkar, C
    Datta, AG
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2003, 245 (1-2) : 43 - 49
  • [3] Can endogenous lipid molecules serve as predictors and prognostic markers of coronary heart disease?
    Das, Undurti N.
    [J]. LIPIDS IN HEALTH AND DISEASE, 2008, 7 (1)
  • [4] Metabolic assessment of human liver transplants from biopsy samples at the donor and recipient stages using high-resolution magic angle spinning 1H NMR spectroscopy
    Duarte, IF
    Stanley, EG
    Holmes, E
    Lindon, JC
    Gil, AM
    Tang, HR
    Ferdinand, R
    McKee, CG
    Nicholson, JK
    Vilca-Melendez, H
    Heaton, N
    Murphy, GM
    [J]. ANALYTICAL CHEMISTRY, 2005, 77 (17) : 5570 - 5578
  • [5] Alterations in myocardial lipid metabolism during myocardial ischemia and reperfusion
    Ford, DA
    [J]. PROGRESS IN LIPID RESEARCH, 2002, 41 (01) : 6 - 26
  • [6] The search for new cardiovascular biomarkers
    Gerszten, Robert E.
    Wang, Thomas J.
    [J]. NATURE, 2008, 451 (7181) : 949 - 952
  • [7] Arachidonic acid and ischemic heart disease
    Hjelte, LE
    Nilsson, Å
    [J]. JOURNAL OF NUTRITION, 2005, 135 (09) : 2271 - 2273
  • [8] JIA GL, 2005, ACTA LAB ANIM SCI SI, V13, P106
  • [9] Metabolic profiling of glucuronides in human urine by LC-MS/MS and partial least-squares discriminant analysis for classification and prediction of gender
    Lutz, Ursula
    Lutz, Roman W.
    Lutz, Werner K.
    [J]. ANALYTICAL CHEMISTRY, 2006, 78 (13) : 4564 - 4571
  • [10] The challenges of modeling mammalian biocomplexity
    Nicholson, JK
    Holmes, E
    Lindon, JC
    Wilson, ID
    [J]. NATURE BIOTECHNOLOGY, 2004, 22 (10) : 1268 - 1274
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