Inter-species interconnections in acid mine drainage microbial communities

被引:53
作者
Comolli, Luis R. [1 ]
Banfield, Jill F. [2 ]
机构
[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Struct Biol & Imaging Dept, Div Life Sci, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Earth & Planetary Sci, Berkeley, CA 94720 USA
基金
美国国家航空航天局;
关键词
Cryo-TEM; Intact natural microbes; Archaea; Microbial inter-species connections; Metagenomics; Thermoplasmatales; ARMAN; NANOARCHAEUM-EQUITANS; IGNICOCCUS-HOSPITALIS; NATURAL COMMUNITIES; BACTERIA; ARCHAEA; RECONSTRUCTION; METABOLISM; INSIGHTS; UNIQUE;
D O I
10.3389/fmicb.2014.00367
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Metagenomic studies are revolutionizing our understanding of microbes in the biosphere. They have uncovered numerous proteins of unknown function in tens of essentially unstudied lineages that lack cultivated representatives. Notably, few of these microorganisms have been visualized, and even fewer have been described ultra-structurally in their essentially intact, physiologically relevant states. Here, we present cryogenic transmission electron microscope (cryo-TEM) 2D images and 3D tomographic datasets for archaeal species from natural acid mine drainage (AMD) microbial communities. Ultrastructural findings indicate the importance of microbial interconnectedness via a range of mechanisms, including direct cytoplasmic bridges and pervasive pili. The data also suggest a variety of biological structures associated with cell-cell interfaces that lack explanation. Some may play roles in inter-species interactions. lnterdependences amongst the archaea may have confounded prior isolation efforts. Overall, the findings underline knowledge gaps related to archaeal cell components and highlight the likely importance of co-evolution in shaping microbial lineages.
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收藏
页数:8
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