Evaluation of Papanicolaou stain for studying micronuclei in buccal cells under field conditions

被引：19
作者：
Ayyad, Sohair B. A.
Israel, Ebenezer
El-Setouhy, Maged
Nasr, Ghada Radwan
Mohamed, Mostafa K.
Loffredo, Christopher A.
机构：
[1] Georgetown Univ, Dept Oncol, Canc Genet & Epidemiol Program, Washington, DC 20007 USA
[2] Ain Shams Univ Hosp, Cytopathol & Early Canc Detect Unit, Cairo, Egypt
[3] Cairo Univ, Dept Community Hlth, Cairo, Egypt
[4] Univ Maryland, Dept Epidemiol & Prevent Med, Baltimore, MD USA
来源：
ACTA CYTOLOGICA | 2006年 / 50卷 / 04期
关键词：
buccal mucosa; Papanicolaou smear; tissue stains; micronucleoli; Papanicolaou stain;
D O I：
10.1159/000325980
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Objective To compare Papanicolaou (Pap) and May-Griinwald Giemsa (MGG) stain as 2 techniques for staining for buccal mucosal cells to detect micronuclei (MN) in field studies. Study Design Eighty cytologic smears (2 per individual) were taken from, the buccal mucosa of 40 cigarette smokers recruited at a rural village in Egypt. Forty smears were stained with Pap stain and 40 with MGG stain. All were assessed for cellularity and scored for MN. Results Pap stain was faster and easier to process and transport in the field study than was MGG stain. Regarding MGG smears, bacteria and cell debris masked the MN as compared to Pap smears, in which the fixative destroyed the bacteria, and made the cell boundaries clearly demarcated. Using Pap stain, MN were seen easily in transparent cytoplasm. Conclusion Pap stain is the preferred method infield studies for scoring and detecting MN in cells of buccal mucosa.
引用
收藏
页码：398 / 402
页数：5
相关论文
共 30 条
[1]   CYTOGENETIC SURVEILLANCE OF TOBACCO-ARECA NUT (MAVA) CHEWERS, INCLUDING PATIENTS WITH ORAL CANCERS AND PREMALIGNANT CONDITIONS [J].
ADHVARYU, SG ;
DAVE, BJ ;
TRIVEDI, AH .
MUTATION RESEARCH, 1991, 261 (01) :41-49
[2]   STANDARDIZATION OF COUNTING MICRONUCLEI - DEFINITION OF A PROTOCOL TO MEASURE GENOTOXIC DAMAGE IN HUMAN EXFOLIATED CELLS [J].
BELIEN, JAM ;
COPPER, MP ;
BRAAKHUIS, BJM ;
SNOW, GB ;
BAAK, JPA .
CARCINOGENESIS, 1995, 16 (10) :2395-2400
[3]   Cytogenetic damage in exfoliated oral mucosal cells and circulating lymphocytes of patients suffering from precancerous oral lesions [J].
Desai, SS ;
Ghaisas, SD ;
Jakhi, SD ;
Bhide, SV .
CANCER LETTERS, 1996, 109 (1-2) :9-14
[4]   Historical perspectives on the development of the in vitro micronucleus test: A personal view [J].
Evans, HJ .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1997, 392 (1-2) :5-10
[5]   The HUman MicroNucleus Project - An international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans [J].
Fenech, M ;
Holland, N ;
Chang, WP ;
Zeiger, E ;
Bonassi, S .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1999, 428 (1-2) :271-283
[6]  
GAREWAL HS, 1993, J CELL BIOCHEM, P206
[7]   Positive correlation between the frequency of micronucleated cells and dysplasia in papanicolaou smears [J].
Guzmán, P ;
Sotelo-Regil, RC ;
Mohar, A ;
Gonsebatt, ME .
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2003, 41 (05) :339-343
[8]  
KEEBLER M, 1991, COMPREHENSIVE CYTOPA
[9]  
Kumar V, 2000, Indian J Dent Res, V11, P101
[10]   Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides:: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells [J].
Lucero, L ;
Pastor, S ;
Suárez, S ;
Durbán, R ;
Gómez, C ;
Parrón, T ;
Creus, A ;
Marcos, R .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2000, 464 (02) :255-262
123