The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple-negative breast cancer cells

被引：5
|
作者：
Pereira De Carvalho, Bruno [1 ]
Chern, Yi-Jye [1 ]
He, Jiabei [1 ]
Chan, Chia-Hsin [1 ,2 ]
机构：
[1] SUNY Stony Brook, Dept Pharmacol Sci, Basic Sci Tower 8-121, Stony Brook, NY 11794 USA
[2] SUNY Stony Brook, Stony Brook Canc Ctr, Stony Brook, NY 11794 USA
来源：
FEBS LETTERS | 2021年 / 595卷 / 02期
关键词：
CDC42; cell migration; cytoskeleton rearrangements; filopodia; focal adhesion; RHOA; RNF8; triple‐ negative breast cancer; DEGRADATION; UBIQUITYLATION; FILOPODIA; SUBSTRATE; GROWTH; CDC42;
D O I：
10.1002/1873-3468.13999
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ubiquitin ligase RNF8 is known to induce epithelial-to-mesenchymal (EMT) transition and metastasis in triple-negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC cells attenuates the protein and mRNA levels of Ras homolog family member A (RHOA) and cell division cycle 42 (CDC42). We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42.
引用
收藏
页码：241 / 252
页数：12
相关论文
共 50 条
  • [21] Investigation of creep properties and the cytoskeletal structures of non-tumorigenic breast cells and triple-negative breast cancer cells
    Onwudiwe, Killian
    Obayemi, John
    Hu, Jingjie
    Oparah, Josephine
    Onyekanne, Chinyerem
    Nwazojie, Chukwudalu
    Aina, Toyin
    Uzonwanne, Vanessa
    Salifu, Ali
    Soboyejo, Winston
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2022, 110 (05) : 1004 - 1020
  • [22] E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer
    Liao, Liqiu
    Song, Mei
    Li, Xin
    Tang, Lili
    Zhang, Tuo
    Zhang, Lixing
    Pan, Yihang
    Chouchane, Lotfi
    Ma, Xiaojing
    CANCER RESEARCH, 2017, 77 (08) : 2090 - 2101
  • [23] Rictor promotes cancer progression of rapamycin-insensitive triple-negative breast cancer cells
    Oneyama, Chitose
    Watanabe, Risayo
    Miyata, Mamiko
    CANCER SCIENCE, 2022, 113 : 1047 - 1047
  • [24] Calsequestrin 2 regulates proliferation, migration, and invasion in triple-negative breast cancer cells
    Kim, Ju Hee
    Hong, Bok Sil
    Heo, Woohang
    Han, Jong Min
    Han, Wonsik
    Noh, Dong-Young
    Moon, Hyeong-Gon
    CANCER RESEARCH, 2018, 78 (13)
  • [25] Transcriptome changes of immune cells across chemotherapy of triple-negative breast cancer
    Gerashchenko, T. S.
    Frolova, A.
    Fedorov, A. A.
    Patysheva, M.
    Vashisth, M.
    Iamshchikov, P.
    Bragina, O.
    Cherdyntseva, N.
    ANNALS OF ONCOLOGY, 2023, 34 : S214 - S214
  • [26] Targeting LRP8 inhibits breast cancer stem cells in triple-negative breast cancer
    Lin, Chang-Ching
    Lo, Miao-Chia
    Moody, Rebecca
    Jiang, Hui
    Harouaka, Ramdane
    Stevers, Nicholas
    Tinsley, Samantha
    Gasparyan, Mari
    Wicha, Max
    Sun, Duxin
    CANCER LETTERS, 2018, 438 : 165 - 173
  • [27] CTSL, a prognostic marker of breast cancer, that promotes proliferation, migration, and invasion in cells in triple-negative breast cancer
    Zhang, Lianmei
    Zhao, Yang
    Yang, Jing
    Zhu, Yaning
    Li, Ting
    Liu, Xiaoyan
    Zhang, Pengfei
    Cheng, Jingliang
    Sun, Suan
    Wei, Chunli
    Fu, Junjiang
    FRONTIERS IN ONCOLOGY, 2023, 13
  • [28] HES1 promotes breast cancer stem cells by elevating Slug in triple-negative breast cancer
    Li, Xiaoying
    Li, Yang
    Du, Xianqiang
    Wang, Xu
    Guan, Shu
    Cao, Yu
    Li, Feng
    Jin, Feng
    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2021, 17 (01): : 247 - 258
  • [29] MLK4 regulates DNA damage response and promotes triple-negative breast cancer chemoresistance
    Dawid Mehlich
    Michał Łomiak
    Aleksandra Sobiborowicz
    Alicja Mazan
    Dagmara Dymerska
    Łukasz M. Szewczyk
    Anna Mehlich
    Agnieszka Borowiec
    Monika K. Prełowska
    Adam Gorczyński
    Paweł Jabłoński
    Ewa Iżycka-Świeszewska
    Dominika Nowis
    Anna A. Marusiak
    Cell Death & Disease, 12
  • [30] MLK4 regulates DNA damage response and promotes triple-negative breast cancer chemoresistance
    Mehlich, Dawid
    Lomiak, Michal
    Sobiborowicz, Aleksandra
    Mazan, Alicja
    Dymerska, Dagmara
    Szewczyk, Lukasz M.
    Mehlich, Anna
    Borowiec, Agnieszka
    Prelowska, Monika K.
    Gorczynski, Adam
    Jablonski, Pawel
    Izycka-Swieszewska, Ewa
    Nowis, Dominika
    Marusiak, Anna A.
    CELL DEATH & DISEASE, 2021, 12 (12)
12345