Extracellular entrapment and degradation of single-walled carbon nanotubes

被引：48
作者：
Farrera, Consol [1 ]
Bhattacharya, Kunal [1 ]
Lazzaretto, Beatrice [1 ]
Andon, Fernando T. [1 ]
Hultenby, Kjell [2 ]
Kotchey, Gregg P. [3 ]
Star, Alexander [3 ]
Fadeel, Bengt [1 ]
机构：
[1] Karolinska Inst, Div Mol Toxicol, Inst Environm Med, S-17177 Stockholm, Sweden
[2] Karolinska Univ, Huddinge Hosp, Karolinska Inst, Dept Lab Med, S-14186 Stockholm, Sweden
[3] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA
来源：
NANOSCALE | 2014年 / 6卷 / 12期
基金：
瑞典研究理事会;
关键词：
ASBESTOS-LIKE PATHOGENICITY; INDUCED TOXICITY FOCUS; STREPTOCOCCUS-PNEUMONIAE; BIODEGRADATION; TRAPS; MYELOPEROXIDASE; MECHANISMS; NEUTROPHILS; CLEARANCE; DNA;
D O I：
10.1039/c3nr06047k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.
引用
收藏
页码：6974 / 6983
页数：10
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