Bioconversion of 5-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336

被引：29

作者：

Wieser, M ^{[1
]}

Heinzmann, K ^{[1
]}

Kiener, A ^{[1
]}

机构：

[1] LONZA AG,DEPT BIOTECHNOL RES,CH-3930 VISP,SWITZERLAND

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1997年 / 48卷 / 02期

关键词：

D O I：

10.1007/s002530051034

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

5-Hydroxypyrazine-2-carboxylic acid, a versatile building block for the synthesis of new antituberculous agents, was prepared by whole-cell biotransformation from 2-cyanopyrazine via pyrazinecarboxylic acid using Agrobacterium sp. DSM 6336. By developing a fermentation process for this two-enzyme-step bioconversion, a product concentration of 286 mM (40 g/l) was obtained. After the isolation method had been optimized the total yield was 80%.

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页码：174 / 176

页数：3

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