Dominant-negative alleles of 14-3-3 proteins cause defects in actin organization and vesicle targeting in the yeast Saccharomyces cerevisiae

被引:53
|
作者
Roth, D [1 ]
Birkenfeld, J [1 ]
Betz, H [1 ]
机构
[1] Max Planck Inst Brain Res, Dept Neurochem, D-60528 Frankfurt, Germany
来源
FEBS LETTERS | 1999年 / 460卷 / 03期
关键词
vesicle trafficking; exocytosis; actin cytoskeleton;
D O I
10.1016/S0014-5793(99)01383-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
14-3-3 Proteins are thought to function as adapters in signaling complexes [1,2], thereby participating in cellular processes including vesicle trafficking and exocytosis [3,4], To delineate further the function of 14-3-3 proteins during vesicle trafficking, we generated dominant-negative alleles of the two 14-3-3 homologues, Bmh1p and Bmh2p, in budding yeast and analyzed their phenotype in respect to exocytosis, Cells overexpressing the carboxy-terminal region of Bmh2p failed to polarize vesicular transport although bulk exocytosis remained unaffected and shelved a disrupted actin cytoskeleton, Our data suggest that 14-3-3 proteins may act primarily on the actin cytoskeleton to regulate vesicle targeting. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:411 / 416
页数:6
相关论文
共 19 条
  • [1] Analysis of dominant-negative alleles of 14-3-3 proteins in the yeast Saccharomyces cerevisiae
    Roth, D
    Betz, H
    MOLECULAR BIOLOGY OF THE CELL, 1998, 9 : 91A - 91A
  • [2] Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae
    Panni, Simona
    Landgraf, Christiane
    Volkmer-Engert, Rudolf
    Cesareni, Gianni
    Castagnoli, Luisa
    FEMS YEAST RESEARCH, 2008, 8 (01) : 53 - 63
  • [3] Regulation of transcription by Saccharomyces cerevisiae 14-3-3 proteins
    Bruckmann, A
    Steensma, HY
    de Mattos, MJT
    van Heusden, GPH
    BIOCHEMICAL JOURNAL, 2004, 382 : 867 - 875
  • [4] Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae
    Kakiuchi, Kazue
    Yamauchi, Yoshio
    Taoka, Masato
    Iwago, Maki
    Fujita, Tomoko
    Ito, Takashi
    Song, Si-Young
    Sakai, Akira
    Isobe, Toshiaki
    Ichimura, Tohru
    BIOCHEMISTRY, 2007, 46 (26) : 7781 - 7792
  • [5] The Saccharomyces cerevisiae 14-3-3 proteins are required for the G1/S transition, actin cytoskeleton organization and cell wall integrity
    Lottersberger, Francisca
    Panza, Andrea
    Lucchini, Giovanna
    Piatti, Simonetta
    Longhese, Maria Pia
    GENETICS, 2006, 173 (02) : 661 - 675
  • [6] Post-transcriptional Control of the Saccharomyces cerevisiae proteome by 14-3-3 proteins
    Bruckmann, Astrid
    Hensbergen, Paul J.
    Balog, Crina I. A.
    Deelder, Andre M.
    Steensma, H. Yde
    van Heusden, G. Paul H.
    JOURNAL OF PROTEOME RESEARCH, 2007, 6 (05) : 1689 - 1699
  • [7] 14-3-3 proteins are essential for regulation of RTG3-dependent transcription in Saccharomyces cerevisiae
    van Heusden, GPH
    Steensma, HY
    YEAST, 2001, 18 (16) : 1479 - 1491
  • [8] 14-3-3 proteins function in the initiation and elongation steps of DNA replication in Saccharomyces cerevisiae
    Yahyaoui, Wafaa
    Zannis-Hadjopoulos, Maria
    JOURNAL OF CELL SCIENCE, 2009, 122 (24) : 4419 - 4426
  • [9] Functions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress
    Lottersberger, F
    Rubert, F
    Baldo, V
    Lucchini, G
    Longhese, MP
    GENETICS, 2003, 165 (04) : 1717 - 1732