共 19 条
Dominant-negative alleles of 14-3-3 proteins cause defects in actin organization and vesicle targeting in the yeast Saccharomyces cerevisiae
被引:53
|作者:
Roth, D
[1
]
Birkenfeld, J
[1
]
Betz, H
[1
]
机构:
[1] Max Planck Inst Brain Res, Dept Neurochem, D-60528 Frankfurt, Germany
来源:
FEBS LETTERS
|
1999年
/
460卷
/
03期
关键词:
vesicle trafficking;
exocytosis;
actin cytoskeleton;
D O I:
10.1016/S0014-5793(99)01383-6
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
14-3-3 Proteins are thought to function as adapters in signaling complexes [1,2], thereby participating in cellular processes including vesicle trafficking and exocytosis [3,4], To delineate further the function of 14-3-3 proteins during vesicle trafficking, we generated dominant-negative alleles of the two 14-3-3 homologues, Bmh1p and Bmh2p, in budding yeast and analyzed their phenotype in respect to exocytosis, Cells overexpressing the carboxy-terminal region of Bmh2p failed to polarize vesicular transport although bulk exocytosis remained unaffected and shelved a disrupted actin cytoskeleton, Our data suggest that 14-3-3 proteins may act primarily on the actin cytoskeleton to regulate vesicle targeting. (C) 1999 Federation of European Biochemical Societies.
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页码:411 / 416
页数:6
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