Peroxidase-like activity of Fe-N-C single-atom nanozyme based colorimetric detection of galactose

被引:68
|
作者
Zhou, Xiaobin [1 ]
Wang, Mengke [1 ]
Chen, Junyang [1 ]
Xie, Xiaolei [1 ]
Su, Xingguang [1 ]
机构
[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China
基金
中国国家自然科学基金;
关键词
Single atom nanozyme; Peroxidase-mimicking activity; Colorimetric detection; Galactose; GOLD; H2O2; NANOPARTICLES; OXIDE; NANOSHEETS; DIAGNOSIS; PLATINUM; REMOVAL; GLUCOSE; SILVER;
D O I
10.1016/j.aca.2020.06.027
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Single atom nanozymes are the artificial enzymes with enzyme-like activity, which have attracted a great deal attention in recent years due to their unique merits such as remarkable stability, excellent atom utilization and low cost. Herein, a convenient and sensitive colorimetric strategy was developed for the sensing of galactose based on Fe-N-C single-atom nanozyme (Fe-SAzyme). The Fe-SAzyme was prepared through "isolation-pyrolysis" method that exhibited intrinsic peroxidase mimicking activity, which can quickly catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue-colored oxTMB in the presence of hydrogen peroxide (H2O2). Galactose can be oxidized by galactose oxidase (Gal Ox) to generate H2O2, and Fe-SAzyme can be utilized for quantitative colorimetric detection of galactose. A good linearity between absorbance and the galactose concentration in the range of 50-500 mu M was obtained with a detection limit of (LOD) 10 mu M. The Fe-SAzyme based colorimetric strategy offered a rapid, convenient and economic way for galactose quantification detection, which could be used as an alternative method for galactosemia diagnosis. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码:72 / 79
页数:8
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