Peptidomic Analysis of Antimicrobial Peptides in Skin Secretions of Amolops mantzorum

被引：6

作者：

Hu, Yuhong ^{[2
]}

Yu, Zhijun ^{[1
]}

Xu, Shiqi ^{[3
]}

Hu, Yonghong ^{[1
]}

Guo, Chao ^{[1
]}

Li, Fengjiao ^{[1
]}

Li, Jing ^{[1
]}

Liu, Jingze ^{[1
]}

Wang, Hui ^{[1
]}

机构：

[1] Hebei Normal Univ, Coll Life Sci, Key Lab Anim Physiol Biochem & Mol Biol Hebei Pro, Shijiazhuang 050024, Hebei, Peoples R China

[2] Hebei Normal Univ, Instrumental Anal Ctr, Shijiazhuang 050024, Hebei, Peoples R China

[3] Chengde Med Univ, Sch Basic Med Sci, Dept Pathogen Biol, Chengde 067000, Peoples R China

来源：

ZOOLOGICAL SCIENCE | 2014年 / 31卷 / 03期

基金：

中国国家自然科学基金;

关键词：

amphibia; antimicrobial peptide; frog skin; Amolops; cDNA library; TORRENT FROG; CYTOLYTIC PEPTIDES; MOLECULAR-CLONING; ACTIVE PEPTIDES; FAMILIES; BREVININ-2; IDENTIFICATION; JINGDONGENSIS; LOLOENSIS; PRECURSOR;

D O I：

10.2108/zsj.31.143

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Amphibian skin secretions contain abundant bioactive peptides that are valuable natural resources for human beings. However, many amphibians are disappearing from the world, making relevant scientific studies even more important. In this study, 24 cDNA sequences encoding antimicrobial peptide (AMP) precursors were initially cloned by screening a cDNA library derived from the skin of the Sichuan torrent frog, Amolops mantzorum. Eighteen mature AMPs belonging to 11 different families were deduced from these cDNA clones. Biological function was confirmed in each family of these AMPs. Some of them were purified from the skin secretions, and their molecular structures were determined by Edman degradation. Liquid chromatography in conjunction with tandem mass spectrometry (LC-MS/MS)-based peptidomics was used to further confirm the actual presence and characteristics of mature AMPs in the skin secretions of A. mantzorum. Incomplete tryptic digestion and gas-phase fractionation (GPF) analysis were used to increase the peptidome coverage and reproducibility of peptide ion selection.

引用

页码：143 / 151

页数：9

共 35 条

[1] Antimicrobial peptides from the skin secretions of the South-East Asian frog Hylarana erythraea (Ranidae) [J].

Al-Ghaferi, Nadia ;

Kolodziejek, Jolanta ;

Nowotny, Norbert ;

Coquet, Laurent ;

Jouenne, Thierry ;

Leprince, Jerome ;

Vaudry, Hubert ;

King, Jay. D. ;

Conlon, J. Michael .

PEPTIDES, 2010, 31 (04) :548-554

[2] ISOLATION AND STRUCTURE OF BOMBESIN AND ALYTESIN, 2 ANALOGOUS ACTIVE PEPTIDES FROM SKIN OF EUROPEAN AMPHIBIANS BOMBINA AND ALYTES [J].

ANASTASI, A ;

ERSPAMER, V ;

BUCCI, M .

EXPERIENTIA, 1971, 27 (02) :166-&

[3]

[Anonymous], 2008, Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts

[4]

Third Informational Supplement. CLSI document M27-S3, P28

[5] The Chinese bamboo leaf odorous frog (Rana (Odorrana) versabilis) and North American Rana frogs share the same families of skin antimicrobial peptides [J].

Chen, Tianbao ;

Zhou, Mei ;

Rao, Pingfan ;

Walker, Brian ;

Shaw, Chris .

PEPTIDES, 2006, 27 (07) :1738-1744

[6] Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami [J].

Chen, Wenlin ;

Yang, Xuening ;

Chen, Lingling ;

Yang, Xiaolong ;

Feng, Feifei ;

He, Weiyu ;

Liu, Jingze ;

Yu, Haining .

BIOCHIMIE, 2011, 93 (07) :1110-1114

[7] Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis [J].

Chen, Zhongming ;

Yang, Xinwang ;

Liu, Zichao ;

Zeng, Lin ;

Lee, Wenhui ;

Zhang, Yun .

BIOCHIMIE, 2012, 94 (02) :328-334

[8]

CLSI, 2008, METH DIL ANT SUSC TE, VEighth, pM07

[9] Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii [J].

Conlon, J. Michael ;

Kolodziejek, Jolanta ;

Nowotny, Norbert ;

Leprince, Jerome ;

Vaudry, Hubert ;

Coquet, Laurent ;

Jouenne, Thierry ;

Iwamuro, Shawichi .

TOXICON, 2007, 50 (06) :746-756

[10] Strategies for transformation of naturally-occurring amphibian antimicrobial peptides into therapeutically valuable anti-infective agents [J].

Conlon, J. Michael ;

Al-Ghaferi, Nadia ;

Abraham, Bency ;

Leprince, Jerome .

METHODS, 2007, 42 (04) :349-357

← 1 2 3 4 →