Clinical significance of Stratifin, ERα and PR promoter methylation in tumor and serum DNA in Indian breast cancer patients

被引：32

作者：

Mirza, Sameer ^{[5
]}

Sharma, Gayatri ^{[5
]}

Parshad, Rajinder ^{[6
]}

Srivastava, Anurag ^{[6
]}

Gupta, Siddartha Datta ^{[7
]}

Ralhan, Ranju ^{[1
,2
,3
,4
,5
]}

机构：

[1] Mt Sinai Hosp, Joseph & Mildred Sonshine Family Ctr Head & Neck, Dept Otolaryngol Head & Neck Surg, Toronto, ON M5G 1X5, Canada

[2] Mt Sinai Hosp, Dept Pathol & Lab Med, Toronto, ON M5G 1X5, Canada

[3] Mt Sinai Hosp, Sonshine Family Ctr Head & Neck Dis, Toronto, ON M5G 1X5, Canada

[4] Univ Toronto, Dept Otolaryngol Head & Neck Surg, Toronto, ON, Canada

[5] All India Inst Med Sci, Dept Biochem, New Delhi 110029, India

[6] All India Inst Med Sci, Dept Surg, New Delhi 110029, India

[7] All India Inst Med Sci, Dept Pathol, New Delhi 110029, India

来源：

CLINICAL BIOCHEMISTRY | 2010年 / 43卷 / 4-5期

关键词：

Breast cancer; DNA methylation; Stratifin; ER alpha; PR; Gene silencing; 14-3-3 SIGMA GENE; CPG ISLANDS; CELL-LINES; HYPERMETHYLATION; ESTROGEN; EXPRESSION; INACTIVATION; CARCINOMA; FREQUENT; CARCINOGENESIS;

D O I：

10.1016/j.clinbiochem.2009.11.016

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: The objective of this study was to determine the concordance of promoter methylation of stratifin, ER alpha and PR in tumor and circulating DNA in breast cancer patients and their association with clinicopathological parameters and disease prognosis. Design and methods: Methylation specific PCR were carried out to investigate the promoter methylation status of stratifin, ERa and PR in tumor and circulating DNA in 100 breast cancer patients in a prospective study. The effect of promoter methylation on protein expression was evaluated by immunohistochemistry. Results: Significant association was observed between promoter methylation of stratifin in tumors (61%) and paired sera (56%) (r = 0.78; p <= 0.001). Loss of stratifin expression was observed in 47% tumors and was associated with poor overall survival (p = 0.05). Significant correlation was observed between methylation status of ERa with PRB (p < 0.0001, OR = 20.8, 95% Cl = 7.4-58.0) and stratifin (p = 0.003, OR = 2.0, 95% Cl = 0.8-4.4). Conclusion: This study underscores the potential utility of serum DNA methylation of these genes as surrogate for tumor DNA methylation as a promising tool for cancer diagnosis. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：380 / 386

页数：7

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