Lipid and carotenoid production by the Rhodosporidium toruloides mutant in cane molasses

被引:34
作者
Zheng, Xuehai [1 ]
Hu, Rongfei [1 ]
Chen, Duo [1 ]
Chen, Jiannan [1 ]
He, Wenjin [1 ]
Huang, Luqiang [1 ]
Lin, Chentao [1 ]
Chen, Huibin [1 ]
Chen, Youqiang [1 ]
Zhu, Jinmao [1 ]
Qi, Feng [1 ]
Xue, Ting [1 ]
机构
[1] Fujian Normal Univ, Coll Life Sci, Southern Inst Oceanog,Fujian Key Lab Special Mari, State Ocean Adm,Publ Serv Platform Industrializat, Fuzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
Rhodosporidium toruloides; Cane molasses; Lipid; Torularhodin; Torulene; beta-carotene; ACCUMULATION; RHODOTORULA;
D O I
10.1016/j.biortech.2021.124816
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Cane molasses is beneficial for lipid and camtenoid production in microalgae. We made a survey for the lipid and carotenoid production profile of R. toruloides M18 (MT) with various concentrations of molasses under nitrogen-deficited conditions. The production of alpha-linolenate and torularhodin from MT were 1.22- and 14.68-fold higher than those of the wild-type strain. We observed that molasses at concentrations of 35 g/L and 70 g/L represented a cheap and environmentally friendly strategy for producing lipids and carotenoids. Transcriptome and WGCNA analysis demonstrated that the genes relevant to the lipid and carotenoid production, including MYB, bHLH, Delta-4 desaturase, Delta-12 desaturase and FA2H, were significantly highly expressed. The results indicated that molasses could represent an inexpensive means for achieving high lipids and camtenoids production in R. toruloides.
引用
收藏
页数:6
相关论文
共 24 条
[1]   MYB118 Represses Endosperm Maturation in Seeds of Arabidopsis [J].
Barthole, Guillaume ;
To, Alexandra ;
Marchive, Chloe ;
Brunaud, Veronique ;
Soubigou-Taconnat, Ludivine ;
Berger, Nathalie ;
Dubreucq, Bertrand ;
Lepiniec, Loic ;
Baud, Sebastien .
PLANT CELL, 2014, 26 (09) :3519-3537
[2]   High lipid accumulation in Yarrowia lipolytica cultivated under double limitation of nitrogen and magnesium [J].
Bellou, S. ;
Triantaphyllidou, I. -E. ;
Mizerakis, P. ;
Aggelis, G. .
JOURNAL OF BIOTECHNOLOGY, 2016, 234 :116-126
[3]   β-Carotene production in sugarcane molasses by a Rhodotorula glutinis mutant [J].
Bhosale, P ;
Gadre, RV .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2001, 26 (06) :327-332
[4]   Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus [J].
Buzzini, Pietro ;
Innocenti, Marzia ;
Turchetti, Benedetta ;
Libkind, Diego ;
van Broock, Maria ;
Mulinacci, Nadia .
CANADIAN JOURNAL OF MICROBIOLOGY, 2007, 53 (08) :1024-1031
[5]   A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3 [J].
Cingolani, Pablo ;
Platts, Adrian ;
Wang, Le Lily ;
Coon, Melissa ;
Tung Nguyen ;
Wang, Luan ;
Land, Susan J. ;
Lu, Xiangyi ;
Ruden, Douglas M. .
FLY, 2012, 6 (02) :80-92
[6]  
Costa Willyan Araújo da, 2020, Braz. J. Food Technol., V23, pe2019064, DOI 10.1590/1981-6723.06419
[7]   OrthoFinder: solving fundamental biases in whole genome comparisons dramatically improves orthogroup inference accuracy [J].
Emms, David M. ;
Kelly, Steven .
GENOME BIOLOGY, 2015, 16
[8]  
Huimin L.I., 2019, FOOD IND, V1, P109
[9]   ELOVL2 overexpression enhances triacylglycerol synthesis in 3T3-L1 and F442A cells [J].
Kobayashi, Tsutomu ;
Zadravec, Damir ;
Jacobsson, Anders .
FEBS LETTERS, 2007, 581 (17) :3157-3163
[10]   WGCNA: an R package for weighted correlation network analysis [J].
Langfelder, Peter ;
Horvath, Steve .
BMC BIOINFORMATICS, 2008, 9 (1)