A new UPLC method for the analysis of Mangiferin in Mangifera indica, Swertia chirayita and Canscora decussate by using Fractional Factorial Design and Its DPPH Scavenging Activity

Mangiferin is a very valuable bio active natural compound that is chemically a xanthonoid and has more significance in pharmacy industry. The main aim of this study was to identify and quantify of mangiferin in Mangifera indica, Swertia chirayita and Canscora decussate and to develop a novel ultra-performance liquid chromatography (UPLC) method. Drug samples were collected from the local market of Dammam, KSA. The mobile phase was used in this study [acetonitrile (ACN): water (50:50 v/v) with isocratic elution with a flow rate of 0.300 mL min-1]. Retention and total run times were 0.680 min and 2.0 min, respectively, with injection volume of 10 mu L at 380 nm. The method was validated for linearity (r2 >= 0.997 +/- 0.0976), accuracy 98.52-102.95%), and RSD of precision (0.7449736-2.284584) with a calibration curve range of 100.00-1200.00 ng/mL. The limits of detection and quantification were found to be 24.68 ng/mL and 74.75 ng/mL, respectively. The fractional factorial design expertwas applied for the validation of robustness and method was found to be robust. The proposed method was found to be accurate, precise and specific and can be applicable for the determination of mangiferin in plant extracts as well as related formulations.